| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We investigated the effect of volatile anesthetics, especially in conditions of high glucose and insulin, on cell proliferation in the human colon or hepatocellular cancer cell lines (HCT116, HT29, and HepG2). First, 1% sevoflurane for 6 h potentially enhanced cell proliferation via KATP channels in colon cancer cells. Second, 1% sevoflurane exposure enhanced cell proliferation in conditions of high glucose (300mg/dL), treated with 0.05 mg/l insulin in HepG2 cells. In addition, we found that 2% sevoflurane for 1 h leads to increased intracellular reactive oxygen species (ROS) concentrations and 200mg/dL of glucose for 3h reduces ROS concentrations potentially via increased osmotic pressure.
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