| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Senescence marker protein-30 (SMP30) / gluconolactonase (GNL) knockout (KO) mice were divided into vitamin C (VC) (+) or VC (-). VC (+) mice were given water containing 1.5 g/L ascorbic acid (AA), whereas VC (-) mice received water without AA. Mice were intraperitoneally injected with lethal dose of lipopolysaccharide (LPS) evaluated physiological parameters and liver and lung injury at 3h after LPS. These results show no significant differences both groups. We speculate that sacrifices after LPS may be too early.
Currently SMP30/ GNL KO mice are receiving water without AA and will become VC (-) mice in October of this year. In next study, high dose of AA or saline will be intraperitoneally administered to VC (-) mice 30 min before lethal dose of LPS injection. At 6-12h after LPS, pituitary-adrenal hormones, oxidative stress markers, inflammatory markers and various organ injuries are going to be measured and evaluated.
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