| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Pregabalin is thought to exert its therapeutic effect in neuropathic pain by inhibiting the activity of spinal dorsal horn(DH) neurons. However, whether a clinical concentration of pregabalin can cause acute inhibition of DH neurons in the spinal cord is controversial. To address this issue, we undertook intracellular Ca2+imaging studies using spinal cord slices, and examined if pregabalin acutely inhibits the primary afferent stimulation-evoked excitation of DH neurons in normal rats and in rats with painful diabetic neuropathy.Clinically relevant(10 μM) and a very high concentration of pregabalin(100 μM) did not affect dorsal roots timulation-evoked Ca2+ signals in normal and diabetic neuropathic rats.These results suggest that the activity of DH neurons in the spinal cord is not inhibited acutely by clinical doses of pregabalin. It is very unlikely that an acute inhibitory action in the DH is the main analgesic mechanism of pregabalin in neuropathic pain states.
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