| Project/Area Number |
26462386
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology
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| Research Institution | Kansai University of Health Sciences |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OHSHIMA Minoru 関西医療大学, 保健医療学部, 准教授 (20342230)
UCHIDA Yasuyuki 関西医療大学, 保健医療学部, 講師 (80388678)
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| Research Collaborator |
KIYOYUKI Yasukuni 関西医療大学, 大学院
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 疼痛 / 下行性ニューロン / 脊髄後角 / パッチクランプ / サブスタンスP / セロトニン / ノルアドレナリン / ラット / 下行性抑制ニューロン / アドレナリン作動性 / セロトニン作動性 / パッチクランプ法 / 侵害情報 / 動物実験 / 疼痛制御機構 |
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| Outline of Final Research Achievements |
Little is known about physiological functions of nociceptive neurons in the deep dorsal horn of spinal cord, as compared with those in the superficial dorsal horn. In this study, deep dorsal horn neurons were examined by using the blind patch clamp technique used freshly sliced spinal cord of the rat. More than half of deep dorsal horn neurons receive substance P (SP) receptors-mediated excitatory inputs, suggesting that these are nociceptive neurons (multireceptive or selectively nociceptive). Of the neurons recorded, about 40% and 20% displayed the slow inward currents by 5-HT and adrenaline, respectively, and these neurons excitatory were activated by substance P. On the other hand, the slow outward currents by 5-HT and adrenaline were observed in a few neurons. The present study suggested that some nociceptive neurons in the deep dorsal horn received 5-HT and adrenaline receptors-mediated excitatory inputs.
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