| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Introduction and Objectives. Intravesical instillation of live bacillus Calmette-Guerin (BCG) is established immuno therapy of non-muscle invasive bladder cancer. Mycolic acids (MA) are the dominant lipids found in the cell wall of Mycobacterium species. We developed novel liposome incorporating MA and examined antitumor effects of MA. Methods. MA was isolated from M. bovis BCG Tokyo, and separated into three subclasses (alpha, methoxy and keto). Liposomes are prepared by sonication and extrusion. C57BL/6 mice were subcutaneously inoculated with a mixture of MB49 cells and PBS or liposome with/without MA followed by additional injection of PBS or liposome. Tumor growth was monitored. Results. Tumor growth was significantly suppressed in mice treated with Keto MA-liposome (p=0.007 vs PBS). Conclusion. Keto MA-liposome showed antitumor effects in mouse.
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