Search for novel therapeutic target molecules focusing on cellular memory in iPS conversion of human kidney cancer cells
| Project/Area Number |
26462400
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
idei mana 東京大学, 医学部附属病院, 特任研究員 (00639213)
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| Co-Investigator(Kenkyū-buntansha) |
菱川 慶一 慶應義塾大学, 医学部(信濃町), 特任准教授 (50255460)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJITA Toshiro 東京大学, 先端科学技術研究所, 特任教授 (10114125)
ABURATANI Hiroyuki 東京大学, 先端科学技術研究所, 特任教授 (10202657)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | iPS細胞 / 初期化 / 再生医学 |
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| Outline of Final Research Achievements |
For the clones whose initialization was confirmed, the difference in DNA methylation was analyzed before and after iPS conversion, and a gene group defining universality and a gene group defining cancer were determined by comparison with human ES cell data . After fiscal year 27, iPS cells derived from human renal carcinoma established in fiscal year 26 were treated exosome purified from the cancer cell culture supernatant and induced to differentiate into cancer stem cells. Next, the induced cancer stem cells were transplanted into SCID mice, and tumors confirmed as cancer stem cells were excised using the proliferative ability as an index, and it was histologically confirmed that they are carcinomas, not teratomas. Finally, the effect on cancer by siRNA treatment or forced expression of the identified gene was examined. From the above, four novel therapeutic target genes have been identified.
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Report
(4 results)
Research Products
(3 results)
