Molecular mechanism of epithelial-mesenchymal transition in castration resistant prostate cancer.

• Project/Area Number: 26462412
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Kobe University
• Principal Investigator: Muramaki Mototsugu 神戸大学, 医学研究科, 医学研究員 (10448178)
• Co-Investigator(Kenkyū-buntansha): 三宅 秀明 浜松医科大学, 医学部, 准教授 (60379435)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 前立腺癌 / 去勢抵抗性前立腺癌 / 上皮間葉移行 / 上皮間葉転換 / クラスタリン

Outline of Final Research Achievements

The Objective of this study was to investigate the significance of epithelial-mesenchymal transition in castration resistant prostate cancer. A study of immunohistochemical staining using clinical tissue samples revealed that N-cadherin was overexpressed in prostate cancer. N-cadherin overexpressed cells showed aggressive features in vitro. N-cadherin overexpressed cells acquired resistance against docetaxel. The acquired resistance was reversed by in vitro treatment using antisense oligonucleaotide against clusterin, one of the stress-induced protein related with anti-apoptotic cell survival in many kinds of cancer cells.

Research Products

• [Journal Article] Antisense Oligonucleotide therapy for patients with castration-resistant prostate cancer (2016)
  • Author(s): Muramaki M,Miyake H,Fujisawa M
  • Journal Title: Nihon Rinsho Volume: 20 Pages: 234-7
  • NAID: 40018884107
• [Journal Article] Assessment of postoperative quality of life: comparative study between laparoscopic and minimum incision endoscopic radical prostatectomies. (2014)
  • Author(s): Muramaki M1, Miyake H, Behnsawy HM, Furukawa J, Harada K, Fujisawa M.
  • Journal Title: Int J Clin Oncol. Volume: 19 Issue: 6 Pages: 10921097-10921097
  • DOI: 10.1007/s10147-013-0659-x
  • Peer Reviewed
• [Presentation] HIF-1 alpha expression in non-muscle invasive urothelial carcinoma of the bladder and its association with EMT markers. (2014)
  • Author(s): 村蒔基次、原田健一、古川順也、三宅秀明、藤澤正人
  • Organizer: 日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27