| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We investigated the role of LDH-A in mediating castration resistance of human prostate cancer (PC) cells by analyzing PC cell lines. LDH expression is strongly associated with docetaxel (DOC) sensitivity in PC cells. A specific LDH-A inhibitor sodium oxamate (SO) inhibited cell growth in PC cells, which was presumably caused by the inhibition of LDH-A protein expression. Synergistic cytotoxicity was observed after the combination therapy with DOC and SO in castration resistant LN-CSS cells, but not in parental LNCaP cells. The combination with DOC and SO caused cell cycle arrest in G2-M and SO promotes DOC-induced apoptosis in LN-CSS cells, which was partially caused by inhibition of DOC-induced increases in LDH-A expression. Our study may provide valuable information for the future development of targeted therapies for CRPC.
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