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The investigation into the novel smooth muscle contraction-relaxation mechanism of lower urinary tract and its clinical application

Research Project

Project/Area Number 26462445
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Urology
Research InstitutionKyushu University

Principal Investigator

Kajioka Shunichi  九州大学, 医学研究院, 准教授 (90274472)

Co-Investigator(Kenkyū-buntansha) 中山 晋介  名古屋大学, 医学系研究科, 准教授 (30192230)
高橋 良輔  九州大学, 大学病院, 助教 (30529805)
松田 美穂  九州大学, 歯学研究院, 講師 (40291520)
逸見 百江  東京医科歯科大学, 大学院医歯学総合研究科, 助教 (40585890)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords過活動膀胱 / 低活動膀胱 / 下部尿路症状 / 尿道平滑筋 / 膀胱排尿筋 / 細胞外カルシウム感知システム / TRPチャネル / トロポニン / カルシウム動態 / カルシウムストア
Outline of Final Research Achievements

This study was aiming at three main targets. Firstly, the contribution of Exchange Protein directly Activated by cAMP (Epac) has been investigated to Ca sensitization of detrusor smooth muscle. In alpha-toxin treated skinned muscle fiber used human detrusor smooth muscle, Epac selective agonist, 8-pCPT-2'-O-Me-cAMP induced little relaxation in the contraction strip induced by 1 microM intracellular Ca. On the other hand, PKA selective cAMP, 6-Bnz-cAMP remarkably induced relaxation by approximately 50%. Taken together, cAMP-induced relaxation in human detrusor was mainly via PKA down stream but not via Epac system. Secondly, the troponin system had been tried to set up in smooth muscle. However, this project had not been successful due to the difficulty of designing transgenic mouse. The final project was to reveal the extracellular Ca and temperature dependency of contraction of urethral smooth muscle. The maximum tension force was obtained at 5mM [Ca]out and 37 celsius degree.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (19 results)

All 2017 2016 2015 2014

All Journal Article (6 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 6 results,  Open Access: 6 results,  Acknowledgement Compliant: 3 results) Presentation (13 results)

  • [Journal Article] Gene polymorphisms in antioxidant enzymes correlate with the efficacy of androgen-deprivation therapy for prostate cancer with implications of oxidative stress.2017

    • Author(s)
      Shiota M, Fujimoto N, Itsumi M, Takeuchi A, Inokuchi J, Tatsugami K, Yokomizo A, Kajioka S, Uchiumi T, Eto M.
    • Journal Title

      Annals of oncology

      Volume: 28(3) Issue: 3 Pages: 569-575

    • DOI

      10.1093/annonc/mdw646

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Micro-coordination of Pacemaker Potentials in the Intestine of the Mouse.2017

    • Author(s)
      Morishita H, Iwata N, Takai C, Mochizuki N, Kaji N, Hori M, Kajioka S, Nakayama S.
    • Journal Title

      Gastroenterology

      Volume: 5085(17) Issue: 8 Pages: 35483-35485

    • DOI

      10.1053/j.gastro.2017.04.016

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Role of PTHrP and Sensory Nerve Peptides in Regulating Contractility of Muscularis Mucosae and Detrusor Smooth Muscle in the Guinea Pig Bladder.2016

    • Author(s)
      Lee K, Mitsui R, Kajioka S, Naito S, Hashitani H.
    • Journal Title

      Journal of Urology

      Volume: 196(4) Issue: 4 Pages: 1287-1291

    • DOI

      10.1016/j.juro.2016.04.082

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Equol inhibits prostate cancer growth via degradation of the androgen receptor by Skp2.2016

    • Author(s)
      Itsumi M, Shiota M, Takeuchi A, Kashiwagi E, Inokuchi J, Tatsugami K, Kajioka S, Uchiumi T, Naito S, Eto M, Yokomizo A.
    • Journal Title

      Cancer Science

      Volume: ◯◯ Issue: 7 Pages: 1022-1028

    • DOI

      10.1111/cas.12948

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Surface vacuolar ATPase in ameloblastoma contributes to tumor invasion of the jaw bone.2016

    • Author(s)
      Yoshimoto S, Morita H, Matsubara R, Mitsuyasu T, Imai Y, Kajioka S, Yoneda M, Ito Y, Hirofuji T, Nakamura S, Hirata M.
    • Journal Title

      International Journal of Oncology

      Volume: 48 (3) Issue: 3 Pages: 1258-1270

    • DOI

      10.3892/ijo.2016.3350

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Actions of cAMP on calcium sensitization in human detrusor smooth muscle contraction.2016

    • Author(s)
      Hayashi M, Kajioka S, Itsumi M, Takahashi R, Shahab N, Ishigami T, Takeda M, Masuda N, Yamaguchi A, Naito S.
    • Journal Title

      British Journal of Urology International

      Volume: 117 (1) Issue: 1 Pages: 179-191

    • DOI

      10.1111/bju.13180

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Is TRP channel involved in warming induced contraction of human and pig urethral smooth muscle?2017

    • Author(s)
      Lee K, Maki T, Takahashi R, Eto M, Kajioka S
    • Organizer
      American Urological Association, Annual Meeting
    • Place of Presentation
      Boston, USA
    • Year and Date
      2017-05-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] 尿道平滑筋における温度、細胞外カルシウム感受性収縮機構2017

    • Author(s)
      李賢、牧知子、高橋良輔、江藤正俊、梶岡俊一
    • Organizer
      日本泌尿器科学会
    • Place of Presentation
      鹿児島
    • Year and Date
      2017-04-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒト膀胱平滑筋における冷却刺激による収縮機構2017

    • Author(s)
      李賢、梶岡俊一、牧知子、高橋良輔、江藤正俊、
    • Organizer
      日本生理学会
    • Place of Presentation
      浜松、静岡
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Does TRP channel play a role in cooling-induced contraction of human detrusor smooth muscle?2017

    • Author(s)
      Kajioka S, Maki T, Lee K, Takahashi R, Eto M
    • Organizer
      European Academy of Urology, Annual Meeting
    • Place of Presentation
      London, UK
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Is TRP channel involved in warming induced contraction of human and pig urethral smooth muscle?2017

    • Author(s)
      牧知子、李賢、高橋良輔、江藤正俊、梶岡俊一
    • Organizer
      日本薬理学会総会
    • Place of Presentation
      長崎
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] 細胞外カルシウムによる尿道平滑筋張力増強のメカニズム2016

    • Author(s)
      牧 知子、梶岡俊一、李 賢、高橋良輔、江藤正俊
    • Organizer
      第93回 日本生理学会大会
    • Place of Presentation
      北海道 札幌市
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Does TRP channel play a role of extracellular calcium sensing in urethral smooth muscle?“2016

    • Author(s)
      Kajioka S, Maki T, Takahashi R, Eto M
    • Organizer
      31st European Urological Association
    • Place of Presentation
      Munich, Germany
    • Year and Date
      2016-03-11
    • Related Report
      2015 Research-status Report
  • [Presentation] 膀胱排尿筋と粘膜筋板における神経性 液性制御の比較特性2015

    • Author(s)
      李 賢 、三井 烈 、橋谷 光、梶岡俊一 、関 成人
    • Organizer
      第 8回 排尿障害モデル動物研究会 排尿障害モデル動物研究会 排
    • Place of Presentation
      静岡県 静岡市
    • Year and Date
      2015-12-04
    • Related Report
      2015 Research-status Report
  • [Presentation] ブタ尿道平滑筋における細胞外カルシウムによる張力増強のメカニズムを探る2015

    • Author(s)
      牧 知子 、梶岡俊一 、高橋良輔 、江藤正俊
    • Organizer
      第 8回 排尿障害モデル動物研究会 排尿障害モデル動物研究会 排
    • Place of Presentation
      静岡県 静岡市
    • Year and Date
      2015-12-04
    • Related Report
      2015 Research-status Report
  • [Presentation] 尿道平滑筋の蓄尿時における張力維持機構の解明2014

    • Author(s)
      林 摩耶
    • Organizer
      第21回日本排尿機能学会
    • Place of Presentation
      岡山
    • Year and Date
      2014-09-17 – 2014-09-20
    • Related Report
      2014 Research-status Report
  • [Presentation] ブタ膀胱排尿筋と尿道平滑筋の収縮弛緩機構の特徴を探る2014

    • Author(s)
      林 摩耶
    • Organizer
      第102回日本泌尿器科学会総会
    • Place of Presentation
      神戸
    • Year and Date
      2014-04-24 – 2014-04-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 下部尿路平滑筋のEpac関連蛋白の分布の検討2014

    • Author(s)
      梶岡 俊一
    • Organizer
      第102回日本泌尿器科学会総会
    • Place of Presentation
      神戸
    • Year and Date
      2014-04-24 – 2014-04-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 夜間頻尿に対するメラトニン受容体アゴニストの有用性と安全性に関する研究2014

    • Author(s)
      尾畑 紘史
    • Organizer
      第102回日本泌尿器科学会総会
    • Place of Presentation
      神戸
    • Year and Date
      2014-04-24 – 2014-04-27
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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