| Project/Area Number |
26462452
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Keio University |
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Principal Investigator |
Asanuma Hiroshi 慶應義塾大学, 医学部(信濃町), 准教授 (70245570)
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| Co-Investigator(Kenkyū-buntansha) |
大家 基嗣 慶應義塾大学, 医学部(信濃町), 教授 (00213885)
香野 日高 東京歯科大学, 歯学部, 講師 (50338133)
水野 隆一 慶應義塾大学, 医学部(信濃町), 講師 (60383824)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 間葉系幹細胞 / 閉塞性腎症 / 閉塞性膀胱障害 / 上皮・間葉系形質転換 / 腎線維化 / 増殖因子 / サイトカイン / 細胞療法 / 上皮間葉系形質転換 |
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| Outline of Final Research Achievements |
The effect of mesenchymal stem cells (MSCs) therapy on chronic obstruction-induced renal fibrosis has not previously been evaluated. Rats with partial ureteral obstruction underwent injection of fluorescent-labeled human and rat MSCs. From 3 days to 4 weeks later, the kidneys were harvested and the renal cortex analyzed for evidence of stem cell infiltration, epithelial-mesenchymal transition (EMT), renal fibrosis, and cytokine and growth factor activity.
MSCs were detected in the interstitium of the kidney more than 4 weeks post-obstruction. MSCs provided protection against obstruction-induced EMT and chronic renal fibrosis. While the mechanism of MSCs-induced renal protection during obstruction remains unclear, exogenous MSCs reduced obstruction-induced TNF-alpha levels, but did not alter TGF-beta1, VEGF, IL-10, FGF, HGF expression.
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