| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Meth A fibro-sarcoma cells transplanted into allogeneic C57Bl/6 mice peritoneal cavities proliferate initially, and then Meth A are rejected out from the mice. The secondary transplantations of Meth A were caused antibody related acute rejections in C57Bl/6 mice. We collected the peritoneal exudate cells (PEC) from these secondary transplantations (S-PEC). And we investigated cytotoxic activity of S-PEC in complement immobilized conditions with 51Cr releasing assays.
We isolated each fractions of S-PEC with FACS cell-sorter, and confirmed the highest allogeneic cytotoxicity was seen in allograft induced macrophages (AIM) fractions. And, we found the activities of AIM were attenuated by massive nonspecific IgG antibodies, in vitro. The removal of AIM in S-PEC also attenuates the cytotoxic activity of S-PEC. It is presumed IVIG acts on cytotoxic activity of AIM. There is a necessity to evaluate the inhibitory effect of IVIG on AIM without compromising complement.
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