| Project/Area Number |
26462471
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
宇都宮 裕貴 東北大学, 大学病院, 准教授 (10359507)
立花 眞仁 東北大学, 大学病院, 講師 (30431571)
坂本 雅弘 東北大学, 大学病院, 技能補佐員 (50645299)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | Basonuclin1 / Bnc1 / SUMO化 / 卵母細胞 / SUMO / NLB / 加齢卵 |
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| Outline of Final Research Achievements |
Using 293 cell line and 10,000 mouse GV stage oocytes, novel Basonuclin1(Bnc1)-binding proteins were identified. It is shown that SUMO E3 ligase Pias4 enhanced sumoylation of Bnc1, which localizes not only nucleoplasm but also Nucleolus-like body (NLB) in young mouse oocyte. Lysin 513 of Bnc1 was specifically modified with SUMO-1, but this sumoylation did not effect the localizational change of Bnc1 in adult GV stage oocyte. Using Bnc1 knockout mouse oocyte, we have shown that Bnc1 regulates folliculogenesis through c-kit signaling pathway.
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