Proteomics analysis of semen obtained from male infertile patients

• Project/Area Number: 26462472
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Tohoku University
• Principal Investigator: Suzuki Kichiya 東北大学, 東北メディカル・メガバンク機構, 非常勤講師 (30422116)
• Co-Investigator(Kenkyū-buntansha): 宇都宮 裕貴 東北大学, 大学病院, 准教授 (10359507) ; 立花 眞仁 東北大学, 大学病院, 講師 (30431571) ; 高橋 藍子 東北大学, 大学病院, 生殖補助医療胚培養士 (30436125)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 生殖医学 / 男性不妊 / 精液 / プロテオミクス / プロテオーム

Outline of Final Research Achievements

We have hypothesized that whether there are factors involved in male infertility with unknown reasons in semen. Therefore we have performed proteome profiling of semen using mass spectrometry (MS) to isolated and determine candidate factors in semen by comparing between healthy volunteers and male infertile patients. Finally we have identified more than two hundreds of protein peaks in semen and chosen approximately twenty candidate molecules, which may involve in male infertility. This result suggested that proteomics analysis of semen using MS is a powerful tool to explore candidate factors related to male infertility and accumulated fundamental knowledge in this field.

Research Products

• [Journal Article] The Tohoku Medical Megabank Project: Design and Mission (2016)
  • Author(s): Kuriyama S, Yaegashi N, et al. (Tamiya G; 11th in 58 authors)
  • Journal Title: Journal of Epidemiology Volume: 26 Issue: 9 Pages: 493-511
  • DOI: 10.2188/jea.JE20150268
  • NAID: 130005409018
  • ISSN: 0917-5040, 1349-9092
  • Peer Reviewed / Open Access
• [Journal Article] The Tohoku Medical Megabank Project: Design and Mission (2016)
  • Author(s): Kuriyama S, Yaegashi N, Nagami F, Arai T, Kawaguchi Y, Osumi N, Sakaida M, Suzuki Y, Nakayama K, Hashizume H, Tamiya G, Kawame H, Suzuki K, the Tohoku Medical Megabank Project Study Group, and Yamamoto M (13 of 57)
  • Journal Title: Journal of Epidemiology Volume: in press
  • NAID: 130005409018
  • Peer Reviewed
• [Journal Article] Fertility and pregnancy outcomes following B-Lynch sutures for post-partum hemorrhage. (2015)
  • Author(s): Tadakawa M, Sugawara J, Saito M, Nishigori H, Utsunomiya H, Nagase S, Tokunaga H, Kurakata-Nakamura M, Sugiyama T, Yaegashi N.
  • Journal Title: J Obstet Gynaecol Res. Volume: 41(4) Issue: 4 Pages: 559-564
  • DOI: 10.1111/jog.12590
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] The effect of Retinoic acid via RARs in endometrial cancer. (2015)
  • Author(s): Keita Tsuji, Hiroki Utsunomiya, Fumihiko Suzuki, Kiyoshi Ito, Nobuo Yaegashi
  • Organizer: Society of Women’s health
  • Place of Presentation: San Francisco （USA)
  • Year and Date: 2015-03-26
  • Invited
• [Presentation] 新規チップ型受精卵呼吸測定装置を用いた受精卵の客観的評価法の検討 (2014)
  • Author(s): 志賀尚美、宇都宮裕貴、高橋藍子、石橋ますみ、黒澤大樹、渡邊善、菅沼亮太、 福井淳史、高橋俊文、阿部宏之、寺田幸弘、八重樫伸生
  • Organizer: 第59回生殖医学会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2014-12-04
  • Invited
• [Presentation] NOVEL AUTOMATEDDEVICE WITH THE CHIP ELECTRODE FR MONITORING RESPIRATORY ACTIVITY OF EMBRYOS. (2014)
  • Author(s): H. Kurosawa, H. UtsunomiyaN. ShigaA. Takahashi, M. Ishibashi, Z. Watanabe, H. Abe,Y.Terada, T. Takahashi, A. Fukui, R. Suganuma,N.Yaegashi.
  • Organizer: ASRM annual meetin
  • Place of Presentation: Hawaii （USA)
  • Year and Date: 2014-10-19
  • Invited