Morphology and functional analysis of mitochondria in uterine leiomyoma
Project/Area Number |
26462475
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Obstetrics and gynecology
|
Research Institution | Chiba University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
生水 真紀夫 千葉大学, 大学院医学研究院, 教授 (30226302)
|
Research Collaborator |
KOBAYASHI Tatsuya
XU Linlin
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 子宮筋腫 / 低酸素 / HIF-1 / ミトコンドリア / 解糖 / HIF1 |
Outline of Final Research Achievements |
Severe hypoxia may cause mitochondrial dysfunction in uterine leiomyoma; therefore, we clarified hypoxic responses in uterine leiomyoma. Expression of hypoxia inducible factor (HIF)-1α was significantly higher in uterine leiomyoma tissues than those in the adjacent myometrium. Hypoxia significantly induced HIF-1α protein expression and intranuclear distribution of HIF-1α in primary cultured uterine leiomyoma cells. Hypoxia also induced recruitment of HIF-1αbinding to the putative HIF response element on the promoter of HIF-responseive genes and subsequent mRNA expression of the HIF-responsive genes in uterine leiomyoma cells. Hypoxia significantly induced apoptosis of uterine leiomyoma cells. Taken together, hypoxia may induce HIF-mediated hypoxic responses in uterine leiomyoma.
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Report
(4 results)
Research Products
(19 results)