| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
In this study, we aimed to establish the model to make the uterine leiomyomas in vivo and to identify molecules involved in the development and progression of uterine leiomyomas. We extracted 2 genes as candidate master gene by DNA methylome analysis and transcriptome analysis. We obtained a cell line in which the gene expression of SATB2 and NRG1 was altered and a cell line in which the expression of both genes was simultaneously altered. Comprehensive gene expression in these cell lines was examined by microarray, and a group of genes whose expression was changed in each cell line was extracted. These gene groups contained a plurality of genes whose expression was changed specifically in the uterine leiomyomas as compared with the normal myometrium. Currently, we are investigating to make the uterine leiomyomas in vivo by xenografts using these cell lines.
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