| Project/Area Number |
26462501
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小出 馨子 昭和大学, 医学部, 助教 (90384437)
四元 淳子 昭和大学, 医学部, 特別研究生 (30553648)
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| Research Collaborator |
Akihiro Kawashima 昭和大学, 医学部, 助教
Takenaka Shin 昭和大学, 医学部, 助教
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 妊娠高血圧症候群 / 妊娠絨毛 / DNAメチル化 / 妊娠初期絨毛 / 喫煙 / 初期絨毛 / エピジェネティクス / 絨毛細胞 / 絨毛細胞分化 |
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| Outline of Final Research Achievements |
We investigated placental DNA methylation in preeclampsia (PE) compared with normal case to understand epigenetic pathogenesis of PE. Placental DNA methylation profiles in 6 weeks, 10-11 weeks, normal cases at third trimester, and cases with PE were made, and they were compared among the groups. In the present study, we targeted CpG sites located on promoters. In comparison of DNA methylation profiles, 246 CpG sites on promoters were detected as the CpG sites that were differentially methylated in PE compared with normal third trimester. The cluster analysis for all samples was done using the 246 CpG sites. We confirmed that not only normal third trimester and PE, but also 6 weeks and 10-11 weeks were classified accurately.
DNA methylation pattern physiologically changes in the villous trophoblasts during the placentation. The insufficiency of placental formation and function leads to the onset of preeclampsia when the change is not sufficient in the villi in early gestation.
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