Investigation on development of therapeutic method for infectious diseases based on microbiome analysis

• Project/Area Number: 26462504
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Aichi Medical University
• Principal Investigator: MIKAMO Hiroshige 愛知医科大学, 医学部, 教授 (00262775)
• Co-Investigator(Kenkyū-buntansha): 山岸 由佳 愛知医科大学, 医学部, 准教授 (60512241)
• Research Collaborator: MATSUMOTO Asami ; IZUMI Koji
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 網羅的細菌叢解析 / 16Sメタゲノム解析法 / 次世代シークエンサー / 腟内細菌叢 / 細菌性腟症 / 早産 / Lactobacillus / ミクロビオータ / 健常女性 / 切迫流・早産 / メタゲノム解析 / クラスター解析 / 16Sメタゲノム解析

Outline of Final Research Achievements

The genus Lactobacillus species prevent invasion and proliferation of pathogenic bacteria in the vaginal flora, while its composition changes with health condition, sexual intercourse and pregnancy. We collected vaginal secretions of non-pregnant women, pregnant women and commercial sex worker (CSW). Vaginal microbiota was analyzed by Terminal Restriction Fragment Length Polymorphism (T-RFLP) method and 16S metagenomic analysis method, and based on the results of vaginal microbiota composition ratios, cluster analysis and diversity analysis was carried out. In addition, vaginal secretions were cultured and the isolation rate of Lactobacillus species was calculated. It has become clear that controlling female vaginal flora by using probiotics and so on for prevention of female genital infections and premature birth could be a new treatment method for infectious diseases.

Research Products

• [Journal Article] 日本人女性腟内細菌叢の網羅的細菌解析 (2017)
  • Author(s): 松本麻未、山岸由佳、和泉孝治、宮本健太郎、岡健太郎、高橋志達、三鴨廣繁
  • Journal Title: 日本臨床腸内微生物学会誌 Volume: 19
  • Peer Reviewed
• [Journal Article] Efficacy and safety of intravenous azithromycin followed by oral azithromycin for the treatment of acute pelvic inflammatory disease and perihepatitis in Japanese women (2014)
  • Author(s): Mikamo H, Iwasaku K, Yamagishi Y, Matsumizu M, Nagashima M
  • Journal Title: J Infect Chemother Volume: 20(7) Issue: 7 Pages: 429-435
  • DOI: 10.1016/j.jiac.2014.04.001
  • Peer Reviewed
• [Presentation] Vaginal Microbiota of Japanese Women (2017)
  • Author(s): Matsumoto A, Yamagishi Y, Miyamoto K, Oka K, Takahashi M, Mikamo H.
  • Organizer: 27 th European Congress of Clinical Microbiology and Infectious Diseases
  • Place of Presentation: オーストリア・ウィーン（Vienna）
  • Int'l Joint Research
• [Presentation] 日本人女性腟内細菌叢の網羅的細菌解析 (2016)
  • Author(s): 松本麻未、山岸由佳、和泉孝治、宮本健太郎、岡健太郎、高橋志達、三鴨廣繁
  • Organizer: 第19回日本臨床腸内微生物学会総会・学術集会
  • Place of Presentation: 東京