Mechanism of inhibitory effect of Carbonyl reductase on cancers aiming to gene therapy
| Project/Area Number |
26462508
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | Carbonyl reductase / Ovarian cancer / アポトーシス / 腫瘍縮小 / 遺伝子治療 / 卵巣癌 / Carbonyl reductase DNA / デンドリマー / TNFα受容体 / Caspase-8 / NF-κB / c-jun |
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| Outline of Final Research Achievements |
Tumor growth was significantly suppressed in mice injected with CR1-overexpressing cells. Tumor volume in the CR1 induction group decreased temporarily until 2 weeks. Tumor cell membranes in both CR1 induction and control groups were positive for TNFR1 expression; however, total protein levels did not differ between the two groups. TNFR-2 expression was comparatively weak in both groups. The expression of NF-κB and c-Jun was weaker in the CR1 induction group than in control. In contrast, caspase-8 and caspase-3 expression was higher in the CR1 induction group. Furthermore, the number of apoptotic cells was significantly greater in tumors that appeared after injections of both types of CR1-overexpressing cells than in those of control cancer cells. These results suggest that CR1 induces apoptosis by activating the caspase pathway via binding to TNFR1.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Contributions of the Japanese Gynecologic Oncology Group (JGOG) in Improving the Quality of Life in Women With Gynecological Malignancies.2017
Author(s)
Futagami F, Yokoyama Y, Shimada M, Sato S, Miyagi E, Tozawa-Ono A, Suzuki N, Fujimura M, Aoki Y, Sagae S, Sugiyama T
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Journal Title
Curr Oncol Rep
Volume: 19
Issue: 4
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] The randomized phase 3 trial of irinotecan plus cisplatin compared with paclitaxel plus carboplatin as first line chemotherapy for ovarian clear cell carcinoma: JGOG3017/GCIG Trial.2016
Author(s)
Sugiyama T, Okamoto A, Enomoto T, Hamano T, Aotani E, Terao Y, Suzuki N, Mikami M, Yaegashi N, Kato K, Yoshikawa H, Yokoyama Y, Tanabe H, Nishino K, Nomura H, Kim JW, Kim BG, Pignata S, Alexandre J, Green J, Isonishi S, Terauchi F, Fujiwara K, Aoki D.
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Journal Title
J Clin Oncol
Volume: 34
Issue: 24
Pages: 2881-2887
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Streptococcal Toxic Shock Syndrome: Life Saving Role of Peritoneal Lavage and Drainage.2016
Author(s)
Yokoyama M, Oyama F, Ito A, Yokota M, Matsukura D, Tsutsumi S, Kasai T, Nitobe Y, Morikawa A, Ozaki T, Yokoyama Y.
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Journal Title
Clin Med Insight Case Rep
Volume: 9
Pages: 75-77
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Clinical efficacy of nedaplatin-based concurrent chemoradiotherapy for uterine cervical cancer: A Tohoku Gynecologic Cancer Unit Study.2016
Author(s)
Kagabu M, Shoji T, Omi H, Honda T, Miura T, Yokoyama Y, Tokunaga H, Takano T, Ohta T, Sato N, Watanabe T, Yamada H, Mizunuma H, Yaegashi N, Nagase S, Terada Y, Fujimori K, Tase T, Sugiyama T.
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Journal Title
Int J Clin Oncol
Volume: 21
Pages: 735-740
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Prognostic role and implications of mutation status of tumor suppressor gene ARID1A in cancer: a systematic review and meta-analysis.2015
Author(s)
Luchini C, Veronese N, Solmi M, Cho H, Kim JH, Chou A, Gill AJ, Faraj SF, Chaux A, Netto GJ, Nakayama K, Kyo S, Lee SY, Kim DW, Yousef GM, Scorilas A, Nelson GS, Köbel M, Kalloger SE, Schaeffer DF, Yan HB, Liu F, Yokoyama Y, Zhang X, Pang D, Lichner Z, Sergi G, Manzato E, Capelli P, Wood LD, Scarpa A, Correll CU.
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Journal Title
Oncotarget
Volume: 36
Issue: 36
Pages: 39088-39097
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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