Establishment of a new tumor marker for trophoblastic neoplasia based on specific sugar chains of hCG
| Project/Area Number |
26462520
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|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
Yamamoto Eiko 名古屋大学, 医学系研究科, 准教授 (10432262)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
柴田 清住 名古屋大学, 医学系研究科, 准教授 (90335026)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Keywords | 絨毛性腫瘍 / 糖鎖 / ｈCG / hCG / 絨毛癌 / 糖転移酵素 |
|---|
| Outline of Final Research Achievements |
Choriocarcinoma cell line with GnT-IVa overexpression (Jar/GnT-IVa) was established and shRNA vectors were made for knockdown of GnT-III expression. GnT-III shRNA vectors were introduced into Jar/GnT-IVa. The levels of sugar chains were not changed by knockdown of GnT-III. Sandwich ELISA was performed using conditioned medium of Jar and GnT-IVa KD (Jar with knockdown of GnT-IVa expression), GSL-II lectin and a mouse antibody.
Whole cell lysate and conditioned medium were collected from Jar/GnT-IVa and control cells. Nano-LC/MS/MS analysis using the proteins showed that LAMP-2 was a target molecule of GnT-IVa.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Molecular hydrogen ameliorates several characteristics of preeclampsia in the Reduced Uterine Perfusion Pressure (RUPP) rat model.2016
Author(s)
Ushida T, Kotani T, Tsuda H, Imai K, Nakano T, Hirako S, Ito Y, Li H, Mano Y, Wang J, Miki R, Yamamoto E, Iwase A, Bando YK, Hirayama M, Ohno K, Toyokuni S, Kikkawa F.
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Journal Title
FREE RADICAL BIOLOGY AND MEDICINE
Volume: 101
Pages: 524-533
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] 寛解に至らなかった高齢発症の臨床的絨毛癌の1例2016
Author(s)
新美 薫, 山本 英子, 藤掛 佳代, 鈴木 史朗, 三輪 陽子, 西野 公博, 中村 謙一, 佐藤 静香, 吉川 史隆
Organizer
第68回日本産婦人科学会学術講演会
Place of Presentation
東京国際フォーラム（東京都千代田区）
Year and Date
2016-04-21
Related Report
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[Presentation] 血尿増悪を繰り返した絨毛癌腎転移症例2016
Author(s)
中村 謙一, 新美 薫, 山本 英子, 藤掛 佳代, 鈴木 史朗, 佐藤 静香, 西野 公博, 三輪 陽子, 吉川 史隆
Organizer
第68回日本産婦人科学会学術講演会
Place of Presentation
東京国際フォーラム（東京都千代田区）
Year and Date
2016-04-21
Related Report
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[Book] 医学書院2016
Author(s)
山本英子
Total Pages
2192
Publisher
今日の治療指針
Related Report
