| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Outline of Final Research Achievements |
We established uterine sarcoma cell lines ;SKN in which CBR1 expressions were increasing stably. In those cell lines, expressions of molecules which has relation with epithelial mesenchymal transition were investigated. Expressions of Fibronectin and N-cadherin were decreasing in CBR1 over expression cells. It is suggested that CBR1 should induce partialy mesenchymal to epithelial transition. But expression of E-cadherin, which is epithelial marker, did not change. CBR1 over expression made their morphologies change to cobble stones from spindle shape. CBR1 over expression made decline MMP-2 in gelatin zymography in SKN. Next, CBR1 over espression clones of SKN was injected to nude mice in order to investigated wheather CBR1 may reduce ability to tumorigenesis.Transplantation of SKN were not able to make tumors in nude mice in 4 weeks.
|
