The effects of ovariotomy on the central and peripheral stress responses

• Project/Area Number: 26462526
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: The University of Tokushima
• Principal Investigator: IWASA Takeshi 徳島大学, 病院, 特任准教授 (00707903)
• Co-Investigator(Kenkyū-buntansha): 苛原 稔 徳島大学, 大学院医歯薬学研究部(医学系), 教授 (20160070) ; 松崎 利也 徳島大学, 大学院医歯薬学研究部(医学系), 准教授 (70294692)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ストレス / 卵巣 / 視床下部 / 脂肪組織 / visfatin / 性腺ホルモン / 心理ストレス / 感染ストレス / サイトカイン

Outline of Final Research Achievements

This study clarified that decrease of serum estradiol level induced by ovariectomy (OVX) increases the central responses to inflammatory stress and that these changes exacerbated febrile response. On the other hand, response to psychological stress was not changed by OVX, indicating that such effects of OVX was specific to inflammatory stress. In addition, this study showed that OVX altered the responses of adipocytokines to inflammatory stress. Because these adipocytokines are related in the stress responses, such changes may be involved in the changes of stress responses induced by OVX.

Research Products

• [Journal Article] Gonadotropin-inhibitory hormone plays roles in stress-induced reproductive dysfunction. (2017)
  • Author(s): Takeshi Iwasa, Toshiya Matsuzaki, Kiyohito Yano, Minoru Irahara
  • Journal Title: Front Endocrine Volume: 8 Pages: 62-62
  • DOI: 10.3389/fendo.2017.00062
  • NAID: 120006550812
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The sensitivity of adipose tissue visfatin mRNA expression to lipopolysaccharide-induced endotoxemia is increased by ovariectomy in female rats. (2016)
  • Author(s): Takeshi Iwasa, Toshiya Matsuzaki, Sumika Matsui, Altankhuu Tungalagsuvd, Munkhsaikhan Munkhzaya, Eri Takiguchi, Takako Kawakita, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara
  • Journal Title: Int Immunopharmacol Volume: 35 Pages: 243-247
  • DOI: 10.1016/j.intimp.2016.04.002
  • Peer Reviewed
• [Journal Article] Effects of chronic testosterone administration on body weight and food intake differ among pre-pubertal, gonadal-intact, and ovariectomized female rats. (2016)
  • Author(s): Takeshi Iwasa, Toshiya Matsuzaki, Altankhuu Tungalagsuvd, Munkhsaikhan Munkhzaya, Mayila Yiliyasi, Takeshi Kato, Akira Kuwahara, and Minoru Irahara
  • Journal Title: Behav Brain Res Volume: 309 Pages: 35-43
  • DOI: 10.1016/j.bbr.2016.04.048
  • Peer Reviewed
• [Journal Article] 電気化学発光免疫測定法(ECLIA法)を用いたエストラジオール測定キット「エクルーシス試薬プロゲステロンIII」の基本性能評価および基準範囲の検討 (2016)
  • Author(s): 松崎利也, 岩佐 武, 徳井貴子, Yiliyasi Mayila, 杉本 光, 苛原 稔
  • Journal Title: 医学と薬学 Volume: 73 Pages: 323-333
• [Journal Article] Developmental changes in hypothalamic toll-like-receptor 4 mRNA expression and the effects of lipopolysaccharide on such changes in female rats. (2015)
  • Author(s): Takeshi Iwasa, Toshiya Matsuzaki, Altankhuu Tungalagsuvd, Munkhsaikhan Munkhzaya, Takako Kawami, Mikio Yamasaki, Masahiro Murakami, Takeshi Kato, Akira Kuwahara, Toshiyuki Yasui, Minoru Irahara
  • Journal Title: Int J Dev Neurosci Volume: 40 Issue: 1 Pages: 12-14
  • DOI: 10.1016/j.ijdevneu.2014.10.002
  • Peer Reviewed
• [Journal Article] The responses of hypothalamic NPY and OBRb mRNA expression to food deprivation develop during the neonatal-prepubertal period and exhibit gender differences in rats. (2015)
  • Author(s): Matsuzaki T, Iwasa T, Tungalagsuvd A, Munkhzaya M, Kawami T, Yamasaki M, Murakami M, Kato T, Kuwahara A, Yasui T, Irahara M.
  • Journal Title: Int J Dev Neurosci. Volume: 41 Issue: 1 Pages: 63-67
  • DOI: 10.1016/j.ijdevneu.2014.11.006
  • Peer Reviewed / Open Access
• [Journal Article] Developmental changes in hypothalamic oxytocin and oxytocin receptor mRNA expression and their sensitivity to fasting in male and female rats. (2015)
  • Author(s): Matsuzaki T, Iwasa T, Munkhzaya M, Tungalagsuvd A, Kawami T, Murakami M, Yamasaki M, Yamamoto Y, Kato T, Kuwahara A, Yasui T, Irahara M.
  • Journal Title: Int J Dev Neurosci. Volume: 41 Issue: 1 Pages: 105-109
  • DOI: 10.1016/j.ijdevneu.2015.01.001
  • Peer Reviewed / Open Access
• [Journal Article] The effects of prenatal undernutrition and postnatal high-fat diet on hypothalamic Kiss1 mRNA and serum leptin levels. (2015)
  • Author(s): Iwasa T, Matsuzaki T, Munkhzaya M, Tungalagsuvd A, Yamasaki M, Kuwahara A, Yasui T, Irahara M.
  • Journal Title: Int J Dev Neurosci. Volume: 42 Issue: 1 Pages: 76-79
  • DOI: 10.1016/j.ijdevneu.2015.03.001
  • Peer Reviewed / Open Access
• [Journal Article] LH and testosterone production are more sensitive to the suppressive effects of food deprivation in prenatally undernourished male rats. (2015)
  • Author(s): Iwasa T, Matsuzaki T, Tungalagsuvd A, Munkhzaya M, Kuwahara A, Yasui T, Irahara M.
  • Journal Title: Int J Dev Neurosci. Volume: 43 Issue: 1 Pages: 66-69
  • DOI: 10.1016/j.ijdevneu.2015.04.001
  • Peer Reviewed
• [Journal Article] Prenatal undernutrition increases the febrile response to lipopolysaccharides in adulthood in male rats. (2015)
  • Author(s): Iwasa T, Matsuzaki T, Tungalagsuvd A, Munkhzaya M, Kuwahara A, Yasui T, Irahara M.
  • Journal Title: Int J Dev Neurosci. Volume: 44 Issue: 1 Pages: 1-5
  • DOI: 10.1016/j.ijdevneu.2015.04.004
  • Peer Reviewed
• [Journal Article] The effects of ovariectomy and LPS-induced endotoxemia on resistin levels in female rats. (2015)
  • Author(s): Takeshi Iwasa, Toshiya Matsuzaki, Sumika Matsui, Altankhuu Tungalagsuvd, Munkhsaikhan Munkhzaya, Takako Kawami, Mikio Yamasaki, Masahiro Murakami, Takeshi Kato, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara
  • Journal Title: Cytokine Volume: 76 Issue: 2 Pages: 558-560
  • DOI: 10.1016/j.cyto.2015.06.003
  • Peer Reviewed
• [Journal Article] The suppressive effect of immune stress on LH secretion is absent in the early neonatal period in rats. (2015)
  • Author(s): Munkhzaya M, Matsuzaki T, Iwasa T, Tungalagsuvd A, Kawami T, Kato T, Kuwahara A, Irahara M.
  • Journal Title: Int J Dev Neurosci. Volume: 46 Issue: 1 Pages: 38-43
  • DOI: 10.1016/j.ijdevneu.2015.06.007
  • Peer Reviewed
• [Journal Article] Developmental changes in the hypothalamic mRNA levels of prepro-orexin and orexin receptors and their sensitivity to fasting in male and female rats. (2015)
  • Author(s): Takeshi Iwasa, Toshiya Matsuzaki, Munkhsaikhan Munkhzaya, Altankhuu Tungalagsuvd, Akira Kuwahara, Toshiyuki Yasui, Minoru Irahara
  • Journal Title: Int J Dev Neurosci Volume: 46 Issue: 1 Pages: 51-54
  • DOI: 10.1016/j.ijdevneu.2015.07.005
  • Peer Reviewed
• [Journal Article] Effects of LPS injection on the hypothalamic and testicular mRNA expression levels of reproductive factors in male rats. (2015)
  • Author(s): 57.Takeshi Iwasa, Toshiya Matsuzaki, Altankhuu Tungalagsuvd, Munkhsaikhan Munkhzaya, Akira Kuwahara, Toshiyuki Yasui, Minoru Irahara
  • Journal Title: Neuroendocrinol Lett Volume: 36 Pages: 193-195
  • Peer Reviewed
• [Journal Article] 電気化学発光免疫測定法(ECLIA法)を用いたエストラジオール測定キット「エクルーシス試薬E2 IV」の基本性能評価および基準範囲の検討 (2015)
  • Author(s): 松崎利也, 岩佐 武, 河見貴子, Mayila Yiliyasi, 高松典通, 土井俊夫, 苛原 稔
  • Journal Title: 医学と薬学 Volume: 72 Pages: 931-941
• [Journal Article] Changes in central and peripheral inflammatory responses to lipopolysaccharide in ovariectomized female rats. (2014)
  • Author(s): Iwasa T, Matsuzaki T, Kinouchi R, Gereltsetseg G, Murakami M, Munkhzaya M, Altankhuu T, Kuwahara A, Yasui T, Irahara M.
  • Journal Title: Cytokine. Volume: 65 Issue: 1 Pages: 65-73
  • DOI: 10.1016/j.cyto.2013.10.004
  • Peer Reviewed
• [Journal Article] Changes in the responsiveness of hypothalamic prokineticin 2 mRNA expression to food deprivation in developing female rats. (2014)
  • Author(s): Iwasa T, Matsuzaki T, Munkhzaya M, Tungalagsuvd A, Kawami T, Murakami M, Yamasaki M, Kato T, Kuwahara A, Yasui T, Irahara M.
  • Journal Title: Int J Dev Neurosci. Volume: 34 Issue: 1 Pages: 76-78
  • DOI: 10.1016/j.ijdevneu.2014.02.001
  • Peer Reviewed
• [Journal Article] Pre-pubertal serum leptin levels and sensitivity to central leptin injection of prenatally undernourished female rats. (2014)
  • Author(s): Iwasa T, Matsuzaki T, Munkhzaya M, Tungalagsuvd A, Kawami T, Murakami M, Yamasaki M, Kato T, Kuwahara A, Yasui T, Irahara M.
  • Journal Title: Int J Dev Neurosci. Volume: 35 Issue: 1 Pages: 52-54
  • DOI: 10.1016/j.ijdevneu.2014.03.007
  • Peer Reviewed
• [Journal Article] Prenatal exposure to glucocorticoids affects body weight, serum leptin levels, and hypothalamic neuropeptide-Y expression in pre-pubertal female rat offspring. (2014)
  • Author(s): Iwasa T, Matsuzaki T, Munkhzaya M, Tungalagsuvd A, Kawami T, Murakami M, Yamasaki M, Kato T, Kuwahara A, Yasui T, Irahara M.
  • Journal Title: Int J Dev Neurosci. Volume: 36 Issue: 1 Pages: 1-4
  • DOI: 10.1016/j.ijdevneu.2014.03.011
  • Peer Reviewed / Open Access
• [Journal Article] Changes in leptin production/secretion induced in response to septic doses of lipopolysaccharides in gonadally intact and ovariectomized female rats. (2014)
  • Author(s): Takeshi Iwasa, Toshiya Matsuzaki, Munkhsaikhan Munkhzaya, Altankhuu Tungalagsuvd, Takako Kawami, Masahiro Murakami, Takeshi Kato, Akira Kuwahara, Toshiyuki Yasui, Minoru Irahara
  • Journal Title: J Reprod Immunol Volume: 104-105 Pages: 92-95
  • DOI: 10.1016/j.jri.2014.03.003
  • Peer Reviewed
• [Journal Article] Site-specific changes in cytokine response to septic dose of lipopolysaccharide in ovariectomized female rats. (2014)
  • Author(s): Takeshi Iwasa, Toshiya Matsuzaki, Ganbat Gereltsetseg, Munkhsaikhan Munkhzaya, Altankhuu Tungalagsuvd, Takako Kawami, Masahiro Murakami, Mikio Yamasaki, Yuri Yamamoto, Akira Kuwahara, Toshiyuki Yasui, Minoru Irahara
  • Journal Title: J Endocrinol Invest Volume: 37 Issue: 8 Pages: 721-727
  • DOI: 10.1007/s40618-014-0094-5
  • Peer Reviewed
• [Journal Article] Hypothalamic Kiss1 and RFRP gene expressions are changed by a high dose of lipopolysaccharide in female rats. (2014)
  • Author(s): Iwasa T, Matsuzaki T, Tungalagsuvd A, Munkhzaya M, Kawami T, Niki H, Kato T, Kuwahara A, Uemura H, Yasui T, Irahara M.
  • Journal Title: Horm Behav. Volume: 66(2) Issue: 2 Pages: 309-316
  • DOI: 10.1016/j.yhbeh.2014.06.007
  • Peer Reviewed / Open Access
• [Journal Article] The effects of LPS-induced endotoxemia on the expression of adiponectin and its receptors in female rats. (2014)
  • Author(s): Takeshi Iwasa, Toshiya Matsuzaki, Sumika Matsui, Munkhsaikhan Munkhzaya, Altankhuu Tungalagsuvd, Takako Kawami, Masahiro Murakami, Takeshi Kato, Akira Kuwahara, Toshiyuki Yasui, Minoru Irahara
  • Journal Title: Endocr J Volume: 61 Pages: 891-900
  • NAID: 130004443985
  • Peer Reviewed
• [Journal Article] Changes in the responsiveness of hypothalamic PK2 and PKR1 gene expression to fasting in developing male rats. (2014)
  • Author(s): Iwasa T, Matsuzaki T, Tungalagsuvd A, Munkhzaya M, Kawami T, Yamasaki M, Murakami M, Kato T, Kuwahara A, Yasui T, Irahara M.
  • Journal Title: Int J Dev Neurosci. Volume: 38 Issue: 1 Pages: 87-90
  • DOI: 10.1016/j.ijdevneu.2014.08.004
  • Peer Reviewed / Open Access
• [Journal Article] 卵巣とストレス反応 (2014)
  • Author(s): 岩佐 武、松崎利也、苛原 稔
  • Journal Title: HORMONE FRONTIER IN GYNECOLOGY Volume: 21 Pages: 273-277
• [Journal Article] アディポサイトカインとゴナドトロピン分泌 (2014)
  • Author(s): 松崎利也、岩佐 武、苛原 稔
  • Journal Title: HORMONE FRONTIER IN GYNECOLOGY Volume: 21 Pages: 199-205
• [Presentation] ストレスが視床下部におけるKiss1およびRFRP遺伝子発現と生殖機能に及ぼす影響 (2016)
  • Author(s): 岩佐 武
  • Organizer: 第69回中国四国産科婦人科学会学術講演会
  • Place of Presentation: サンポートホール高松(香川県高松市)
  • Year and Date: 2016-09-24
• [Presentation] 雌ラットにおいてテストステロンが体重・摂食量に及ぼす影響は性腺ホルモン環境により異なる (2016)
  • Author(s): 岩佐 武、松崎利也、Munkhsaikhan Munkhzaya、Altankhuu Tungalagsuvd、馬依拉 伊利亜斯、苛原 稔
  • Organizer: 第68回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京国際フォーラム(東京都千代田区)
  • Year and Date: 2016-04-22
• [Presentation] Hypothalamic Kiss1 and RFRP gene expressions are changed by a high dose of lipopolysaccharide in female rats. (2015)
  • Author(s): Takeshi Iwasa, Toshiya Matsuzaki, Takako Kawami, Munkhsaikhan Munkhzaya, Altankhuu Tungalagsuvd, Minoru Irahara
  • Organizer: IFFS International Meeting in Yokohama, Japan
  • Place of Presentation: Yokohama, Japan, PacificoYokohama
  • Year and Date: 2015-04-26
  • Int'l Joint Research
• [Presentation] Suppressive effect of immune stress on LH secretion is absent in early neonatal period in rats. (2015)
  • Author(s): Munkhsaikhan Munkhzaya, Toshiya Matsuzaki, Takeshi Iwasa, Altankhuu Tungalagsuvd, Takako Kawami, Minoru Irahara
  • Organizer: IFFS International Meeting in Yokohama, Japan
  • Place of Presentation: Yokohama, Japan, PacificoYokohama
  • Year and Date: 2015-04-26
  • Int'l Joint Research
• [Presentation] Senktide does not recover suppression of HPG axis induced by acute fasting in male rats. (2015)
  • Author(s): Altankhuu Tnugalagsuvd, Toshiya Matsuzaki, Takeshi Iwasa, Munkhsaikhan Munkhzaya, Takako Kawami, Minoru Irahara
  • Organizer: IFFS International Meeting in Yokohama, Japan
  • Place of Presentation: Yokohama, Japan, PacificoYokohama
  • Year and Date: 2015-04-26
  • Int'l Joint Research
• [Presentation] Comparison of AMH and FSH as an indicator of ovarian reserve in ART. (2015)
  • Author(s): Mikio Yamasaki, Akira Kuwahara, Yuka Taniguchi, Yuri Yamamoto, Takeshi Iwasa, Kenji Hinokio, Toshiya Matsuzaki, Minoru Irahara
  • Organizer: IFFS International Meeting in Yokohama, Japan
  • Place of Presentation: Yokohama, Japan, PacificoYokohama
  • Year and Date: 2015-04-26
  • Int'l Joint Research
• [Presentation] Clinical significance of estradiol and transvaginal ultrasonography for pregnancy outcomes in natural frozen-thawed blastcyst transfer cycles. (2015)
  • Author(s): Akira Kuwahara, Kenji Hinokio, Yuka Taniguchi, Yuri Yamamoto, Mikio Yamasaki, Takeshi Iwasa, Minoru Irahara
  • Organizer: IFFS International Meeting in Yokohama, Japan
  • Place of Presentation: Yokohama, Japan, PacificoYokohama
  • Year and Date: 2015-04-26
  • Int'l Joint Research
• [Presentation] Survey on oncologist and gynecologist concerned with fertility for cancer survivors in reproductive age. (2015)
  • Author(s): Akiko Abe, Masato Nishimura, Eri Takiguchi, Takako Kawakita, Yuka Taniguchi, Yuri Yamamoto, Mikio Yamasaki, Takeshi Iwasa, Akira Kuwahara, Toshiya Matsuzaki, Minoru Irahara
  • Organizer: IFFS International Meeting in Yokohama, Japan
  • Place of Presentation: Yokohama, Japan, PacificoYokohama
  • Year and Date: 2015-04-26
  • Int'l Joint Research
• [Presentation] Efficacy and pregnant outcomes of fertility-sparing treatment with medroxyprogesterone acetate for endometrial carcinoma and atypical endometrial hyperplasia in young women. (2015)
  • Author(s): Masato Nishimura, Eri Takiguchi, Takako Kawakita, Akiko Abe, Yuka Taniguchi, Yuri Yamamoto, Mikio Yamasaki, Takeshi Iwasa, Akira Kuwahara, Toshiya Matsuzaki, Minoru Irahara
  • Organizer: IFFS International Meeting in Yokohama, Japan
  • Place of Presentation: Yokohama, Japan, PacificoYokohama
  • Year and Date: 2015-04-26
  • Int'l Joint Research
• [Presentation] 卵巣摘出が中枢・末梢のストレス反応に与える影響 (2015)
  • Author(s): 岩佐 武、松崎利也、苛原 稔
  • Organizer: 第8回日本性差医学・医療学会学術集会
  • Place of Presentation: ホテルクレメント徳島(徳島県徳島市)
  • Year and Date: 2015-01-31
• [Presentation] 卵巣摘出による中枢・末梢のストレス反応の変化 (2014)
  • Author(s): 岩佐 武、松崎利也、苛原 稔
  • Organizer: 第87回日本内分泌学会
  • Place of Presentation: 福岡国際会議場(福岡県福岡市)
  • Year and Date: 2014-04-25
• [Presentation] 卵巣摘出が中枢・末梢のストレス反応に及ぼす影響 (2014)
  • Author(s): 岩佐 武、松崎利也、苛原 稔
  • Organizer: 第66回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京国際フォーラム(東京都千代田区)
  • Year and Date: 2014-04-18