| Project/Area Number |
26462528
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Ehime University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小泉 雅江 愛媛大学, 医学系研究科, 助教 (60583028)
那波 明宏 名古屋大学, 医学系研究科, 特任教授 (90242859)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 腫瘍発生学 / 子宮頸癌 / 動物モデル / トランスジェニックマウス |
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| Outline of Final Research Achievements |
In this study, we examined the impact of elevated several growth factors/oncogenes in cervical epithelium of transgenic mice. We generated transgenic mice those overexpressed transforming growth factor-a (TGF-α), c-src or insulin-like growth factor-1 (IGF-1) in epithelial basal cells driven by the keratin 14 (K14) or keratin 5 (K5) promoter. Histopathologic changes were assessed in uterine cervix of 12-month-old TGF-α transgenic mice, Src transgenic mice and IGF-1 transgenic mice, compared to age-matched non-transgenic littermates. The incidence of cervical intraepithelial neoplasia 3 (CIN3) was 37.5% (6/16), 30% (6/20) and 20.8% (5/24) in TGF-α transgenic mice, src transgenic mice and IGF-1 transgenic mice, respectively. Constitutive expression of growth factors/oncogenes, including TGF-α, c-src and IGF-1, and activation of the epidermal growth factor receptor in epithelium of uterine cervix may play an important role in tumorigenesis in this tissue.
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