A novel regulatory mechanism of endometrial cancer invasion and metastasis by transcription factors

• Project/Area Number: 26462529
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Kyushu University
• Principal Investigator: ASANOMA Kazuo 九州大学, 医学研究院, 准教授 (30380413)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 子宮内膜癌 / 上皮間葉移行 / 転写因子

Outline of Final Research Achievements

In the present study, we demonstrated that the expression of DEC1 and DEC2 was controlled in a pathological stage-dependent manner in human endometrial cancer (HEC). Our in vitro assays showed that DEC1 and DEC2 suppressed tumor cell invasion. DEC1 and DEC2 also suppressed the transcription of the EMT effector, TWIST1. We elucidated that the transcription factor SP1 was involved in the basal transcriptional activity of TWIST1 and that DEC1 and DEC2 competed with SP1 for DNA binding to regulate the gene transcription. We presented these results as a paper (Asanoma et al., 2015).

Research Products

• [Journal Article] GEP oncogene promotes cell proliferation through YAP activation in ovarian cancer (2016)
  • Author(s): Yagi H, Asanoma K, Ohgami T, Ichinoe A, Sonoda K, Kato K.
  • Journal Title: Oncogene Volume: 10 Issue: 34 Pages: 1-10
  • DOI: 10.1038/onc.2015.505
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] FBXW7 is involved in the acquisition of the malignant phenotype in epithelial ovarian tumors. (2016)
  • Author(s): Kitade S, Onoyama I, Kobayashi H, Yagi H, Yoshida S, Kato M, Tsunematsu R, Asanoma K, Sonoda K, Wake N, Hata K, Nakayama KI, Kato K
  • Journal Title: Cancer Science Volume: 107 Issue: 10 Pages: 1399-1405
  • DOI: 10.1111/cas.13026
  • Peer Reviewed
• [Journal Article] SNP55, a new functional polymorphism of MDM2-P2 promoter, contributes to allele-specific expression of MDM2 in endometrial cancers. (2015)
  • Author(s): Okamoto K, Tsunematsu R, Tahira T, Sonoda K, Asanoma K, Yagi H, Yoneda T, Hayashi K, Wake N, Kato K
  • Journal Title: BMC Medical Genetics Volume: 16 Issue: 1 Pages: 67-67
  • DOI: 10.1186/s12881-015-0216-8
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of the Mechanism of TWIST1 Transcription by BHLHE40 and BHLHE41 in Cancer Cells. (2015)
  • Author(s): Asanoma K, Liu G, Yamane T, Miyanari Y, Takao T, Yagi H, Ohgami T, Ichinoe A, Sonoda K, Wake N, Kato K
  • Journal Title: Molecular and Cellular Biology Volume: 35 Issue: 24 Pages: 4096-4109
  • DOI: 10.1128/mcb.00678-15
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of the Critical Site of Calponin 1 for Suppression of Ovarian Cancer Properties. (2015)
  • Author(s): Yamane T, Asanoma K, Kobayashi H, Liu G, Yagi H, Ohgami T, Ichinoe A, Sonoda K, Wake N, Kato K
  • Journal Title: Anticancer Research Volume: 35 Pages: 5993-5999
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Aryl hydrocarbon receptor SNP -130 C/T associates with dioxins susceptibility through regulating its receptor activity and downstream effectors including interleukin 24 (2015)
  • Author(s): Liu G, Asanoma K, Takao T, Tsukimori K, Uchi H, Furue M, Kato K, Wake N
  • Journal Title: Toxicology Letters. Volume: 232 Issue: 2 Pages: 384-392
  • DOI: 10.1016/j.toxlet.2014.11.025
  • Peer Reviewed / Open Access
• [Journal Article] Uterine myxoid leiomyosarcoma with tumor embolism extending into the right atrium (2015)
  • Author(s): Hiromi Imai, Hiroshi Yagi, Kaoru Okugawa, Hironori Kenjo, Tatsuhiro Ohgami, Yoshiaki Kawano, Eisuke Kaneki, Akimasa Ichinoe, Kazuo Asanoma, Hideaki Yahata, Kenzo Sonoda, Hiroaki Kobayashi, Tsunehisa Kaku, Kiyoko Kato
  • Journal Title: Case Reports in Obstetrics and Gynecology Volume: 2015 Pages: 316262-316262
  • DOI: 10.1155/2015/316262
  • Peer Reviewed / Open Access
• [Presentation] BHLHE40 and BHLHE41 suppress epithelial-to-mesenchymal transition of uterine endometrial cancer cells by inhibiting SP1 function. (2016)
  • Author(s): ASANOMA kazuo
  • Organizer: 第75回日本癌学会
  • Place of Presentation: 横浜
  • Year and Date: 2016-10-08
• [Presentation] BHLHE40 and BHLHE41 suppress epithelial-to-mesenchymal transition of uterine endometrial cancer cells by inhibiting SP1 function. (2016)
  • Author(s): ASANOMA kazuo
  • Organizer: 第58回日本婦人科腫瘍学会
  • Place of Presentation: 米子
  • Year and Date: 2016-07-09
• [Presentation] Basic HLH type of transcriptional factors, BHLHE40 and BHLHE41 suppress epithelial-to-mesenchymal transition of uterine endometrial cancer cells by inhibiting SP1 function. (2016)
  • Author(s): ASANOMA kazuo
  • Organizer: 第68回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2016-04-22
• [Presentation] BasicHLHtypeoftranscriptionalfactors, DEC1andDEC2suppressepithelial-to-mesenchymal transitionofuterineendometrialcancercells. (2015)
  • Author(s): Kazuo Asanoma
  • Organizer: 67th Annual Congress of the Japan Society of Obstetrics and Gynecology
  • Place of Presentation: Yokohama
  • Year and Date: 2015-04-10
• [Presentation] Transcriptional factors, DEC1 and DEC2 cooperatively regulate epithelial-to-mesenchymal transition of uterine endometrial cancer cells. (2014)
  • Author(s): ASANOMA Kazuo, KOBAYASHI Hiroaki, WAKE Norio, KATO Kiyoko
  • Organizer: The 67th Annual Congress of Japan Society of Obstetrics & Gynecology
  • Place of Presentation: 東京
  • Year and Date: 2014-04-18 – 2014-04-20