| Project/Area Number |
26462540
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Fujita Health University |
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Principal Investigator |
Fujii Takuma 藤田保健衛生大学, 医学部, 教授 (10218969)
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| Co-Investigator(Kenkyū-buntansha) |
岩田 卓 慶應義塾大学, 医学部(信濃町), 講師 (30296652)
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| Co-Investigator(Renkei-kenkyūsha) |
Takayanagi Atsushi 慶應義塾大学, 医学部, 講師 (80245464)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | human papillomavirus / vaccine / cervical cancer / 子宮頸癌 / ワクチン / ヒトパピローマウイルス / 子宮頸がん / パピローマウイルス / マイクロRNA / サイトカイン |
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| Outline of Final Research Achievements |
Monitoring the attribution of human papillomavirus (HPV) genotypes to cervical precancerous lesions is essential for evaluating the potential efficacy of HPV vaccines. Three procedures, Clinichip HPV test (Chip) and modified GP5+/6+ PCR coupled to fluorescent bead sorter detection (MGP) in exfoliated cervical cells (C-Chip and C-MGP, respectively) or formalin-fixed paraffin-embedded tissues (F-MGP), were compared to determine the appropriate genotyping procedures. In CIN 2/3 specimens, HPV16/18/31/33/45/52/58 hierarchical attribution was 88.4% (76/86) with C-Chip, 86.0% (74/86) with C-MGP, and 83.7% (72/86) with F-MGP (p = 0.49). Although F-MGP is theoretically a reliable method for determining HPV genotype attribution, it is acceptable to use C-Chip or C-MGP, coupled to the hierarchical attribution formula to correct the bias of multiple infections. These approaches using exfoliated cervical cells are practical for monitoring the efficacy of HPV vaccines.
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