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Reconstitution of gynecological cancer microenvirnment

Research Project

Project/Area Number 26462542
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKawasaki Medical School

Principal Investigator

Murata Takuya  川崎医科大学, 医学部, 講師 (20714207)

Co-Investigator(Kenkyū-buntansha) 中村 隆文  川崎医科大学, 医学部, 教授 (20303969)
冨松 拓治  大阪大学, 医学系研究科, 講師 (30346209)
下屋 浩一郎  川崎医科大学, 医学部, 教授 (40291950)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsがんの微小環境 / 癌関連線維芽細胞 / 子宮頸癌 / 細胞間相互作用 / 蛍光細胞標識 / リンパ節転移 / 癌の微小環境 / リンパ節転移遺伝子 / 婦人科がん治療 / ヌードマウス同所移植 / テーラーメイドがん治療 / がん組織バンク / 希少婦人科がん
Outline of Final Research Achievements

Reconstitution of cancer microenvirnment in vitro and/or in vivo is still difficult to achieve. If complete reconstitution of cancer microenvirnment be possible, it will be the break through to new cancer treatment, making cancer drug screening easier and find new biological mechanism that can be a new treatment target. In the course of the study, we found that cancer-associated fibroblast can make ME180, cervial caner cell line, metastasize to lymph nodes at the rate of as high as 40%, by co-transplanting these two kind of cells in nude mouse subcutaneously, that never occured if single kind of cancer cell was transplanted. By further studying this phenomenon, it may be possible to reconstitute cervical cancer microenvirnment in nude mouse subcutaneous completely.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015 2014

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (5 results)

  • [Journal Article] Reconstitution of a metastatic-resistant tumor microenvironment with cancer-associated fibroblasts enables metastasis.2017

    • Author(s)
      Murata T, Mekada E, Hoffman RM.
    • Journal Title

      Cell Cycle.

      Volume: 16 Issue: 6 Pages: 533-535

    • DOI

      10.1080/15384101.2017.1281486

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cervical Cancer Patient-Derived Orthotopic Xenograft (PDOX) Is Sensitive to Cisplatinum and Resistant to Nab-paclitaxel.2017

    • Author(s)
      Murakami T, Murata T, Kawaguchi K, Kiyuna T, Igarashi K, Hwang HK, Hiroshima Y, Hozumi C, Komatsu S, Kikuchi T, Lwin TM, Delong JC, Miyake K, Zhang Y, Tanaka K, Bouvet M, Endo I, Hoffman RM.
    • Journal Title

      Anticancer Res.

      Volume: 37 Pages: 61-65

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Invasion of uterine cervical squamous cell carcinoma cells is facilitated by locoregional interaction with cancer-associated fibroblasts via activating transforming growth factor-beta.2015

    • Author(s)
      Michikazu Nagura, Noriomi Matsumura, Tsukasa Baba, Ryusuke Murakami, Budiman Kharma, Junzo Hamanishi, Ken Yamaguchi, Kaoru Abiko, Masafumi Koshiyama, MasakiMandai, TakuyaMurata, Susan K. Murphy, Ikuo Konishi
    • Journal Title

      Gynecologic Oncology

      Volume: 136 Pages: 104-111

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment of a Patient-Derived Orthotopic Xenograft (PDOX) Model of HER- 2-Positive Cervical Cancer Expressing the Clinical Metastatic Pattern.2015

    • Author(s)
      Yukihiko Hiroshima, Yong Zhang, Nan Zhang, Ali Maawy, Sumiyuki Mii, Mako Yamamoto, Fuminari Uehara, Shinji Miwa, Shuya Yano, Takashi Murakami, Masashi Momiyama, Takashi Chishima, Kuniya Tanaka, Yasushi Ichikawa, Michael Bouvet, Takuya Murata, Itaru Endo, Robert M. Hoffman
    • Journal Title

      PLOS ONE

      Volume: 10

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 子宮頸癌関連線維芽細胞の癌増殖促進遺伝子解析2017

    • Author(s)
      村田卓也
    • Organizer
      第69回日本産科婦人科学会学術講演会
    • Place of Presentation
      広島県広島市 広島グリーンアリーナ
    • Related Report
      2016 Annual Research Report
  • [Presentation] 子宮頸癌の微小環境における癌関連線維芽細胞の作用2017

    • Author(s)
      村田卓也
    • Organizer
      第38回癌免疫外科研究会
    • Place of Presentation
      岡山県倉敷市 倉敷アイビースクエア
    • Related Report
      2016 Annual Research Report
  • [Presentation] 子宮頸癌における癌関連線維芽細胞に起因したリンパ節転移因子の同定2016

    • Author(s)
      村田卓也
    • Organizer
      第7回川崎医科大学学術集会
    • Place of Presentation
      岡山県倉敷市 川崎医科大学
    • Related Report
      2016 Annual Research Report
  • [Presentation] 子宮頸癌における癌微小環境細胞と癌細胞の相互作用解析2015

    • Author(s)
      村田卓也
    • Organizer
      第6回川崎医科大学学術集会
    • Place of Presentation
      川崎医科大学8階ポスター発表会場(岡山県・倉敷市)
    • Year and Date
      2015-08-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Uterine cervical cancer-associated fibroblasts enhance lymph node metastasis in nude mouse models of cervical cancer.2014

    • Author(s)
      Takuya Murata, Hiroto Mizushima, Robert Hoffman, Eisuke Mekada, Takafumi Nakamura
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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