Microbiological analysis of Streptococcus pneumoniae in middle ear fluid isolates on acute otitis media in Japanese children after seven-valent pneumococcal conjugate vaccine.

• Project/Area Number: 26462546
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Tohoku University
• Principal Investigator: okitsu naohiro 東北大学, 医学系研究科, 非常勤講師 (20375059)
• Co-Investigator(Kenkyū-buntansha): 矢野 寿一 奈良県立医科大学, 医学部, 教授 (20374944) ; 日高 浩史 東北大学, 医学系研究科, 准教授 (40302103)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 7価結合型肺炎球菌ワクチン / 急性中耳炎 / 血清型 / 薬剤感受性 / 質量分析

Outline of Final Research Achievements

We surveyed the serotypes and antimicrobial susceptibility profile of 176 S. pneumoniae isolates obtained from the middle ear fluid of Japanese children with AOM between April and September 2013, aim to evaluate the impact of seven-valent pneumococcal conjugate vaccine (PCV7). Serotype 19A, 15A and 3 showed a high frequency and we revealed the serotype replacement of PCV7. Serotype 15A is not included in second-generation vaccine (13-valent pneumococcal conjugate vaccine), and all isolates of serotype 15A in this study showed multi-drug resistance. Furthermore, by multilocus sequence typing, we revealed all serotype 15A isolates exept one was included in clonal complex 63 and was genetically close.

Research Products

• [Journal Article] Predicting postoperative fever and bacterial colonization on packing material following endoscopic endonasal surgery. (2017)
  • Author(s): Nomura K, Yamanaka Y, Sekine Y, Yamamoto H, Esu Y, Hara M, Hasegawa M, Shinnabe A, Kanazawa H, Kakuta R, Ozawa D, Hidaka H, Katori Y, Yoshida N.
  • Journal Title: Eur Arch Otorhinolaryngol. Volume: 274 Issue: 1 Pages: 167-73
  • DOI: 10.1007/s00405-016-4189-9
  • Peer Reviewed
• [Journal Article] Molecular Epidemiology of Ampicillin-resistant Haemophilus influenzae Causing Acute Otitis Media in Japanese Infants and Young Children. (2016)
  • Author(s): Kakuta R, Yano H, Hidaka H, Kanamori H, Endo S, Ichimura S, Ogawa M, Shimojima M, Ozawa D, Inomata S, Tanouchi A, Kaku M, Katori Y.
  • Journal Title: Pediatr Infect Dis J. Volume: 35 Issue: 5 Pages: 501-6
  • DOI: 10.1097/inf.0000000000001066
  • Peer Reviewed
• [Journal Article] First report of severe acute otitis media caused by Campylobacter rectus and review of the literature. (2016)
  • Author(s): Kakuta R, Hidaka H, Yano H, Okamoto M, Ozawa D, Endo S, Kaku M, Katori Y.
  • Journal Title: J Infect Chemother. Volume: 22 Issue: 12 Pages: 800-3
  • DOI: 10.1016/j.jiac.2016.06.001
  • Peer Reviewed
• [Journal Article] Impact of the 7-valent pneumococcal conjugate vaccine on acute otitis media in Japanese children: Emergence of serotype 15A multi-drug resistant Streptococcus pneumoniae in middle ear fluid isolates. (2015)
  • Author(s): Ozawa D, Yano H, Endo S, Hidaka H, Kakuta R, Okitsu N, Kanamori H, Ogawa M, Ichimura S, Shimojima M, Inomata S, Kaku M, and Katori Y.
  • Journal Title: Pediatr Infect Dis J. Volume: 34 Issue: 9 Pages: 217-21
  • DOI: 10.1097/inf.0000000000000776
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prevalence of Smqnr and plasmid-mediated quinolone resistance determinants in clinical isolates of Stenotrophomonas maltophilia from Japan: novel variants of Smqnr. (2015)
  • Author(s): Kanamori H, Yano H, Tanouchi A, Kakuta R, Endo S, Ichimura S, Ogawa M, Shimojima M, Inomata S, Ozawa D, Aoyagi T, Weber DJ, Kaku M.
  • Journal Title: New Microbes New Infect. Volume: 7 Pages: 8-14
  • DOI: 10.1016/j.nmni.2015.04.009
  • Peer Reviewed
• [Journal Article] Outcomes of frontal mucoceles treated with conventional endoscopic sinus surgery. (2015)
  • Author(s): Nomura K, Hidaka H, Arakawa K, Sugawara M, Ozawa D, Okumura Y, Takata Y, Katori Y.
  • Journal Title: Acta Otolaryngol. Volume: 135 Issue: 8 Pages: 819-23
  • DOI: 10.3109/00016489.2015.1021933
  • Peer Reviewed
• [Journal Article] Clinical factors indicating short nasal bone overlap. (2015)
  • Author(s): Nomura Y, Nomura K, Ozawa D, Arakawa K, Hidaka H, Katori Y.
  • Journal Title: Acta Otolaryngol. Volume: 135 Issue: 11 Pages: 1196-9
  • DOI: 10.3109/00016489.2015.1061698
  • Peer Reviewed
• [Journal Article] Microbiological and molecular epidemiological analyses of community-associated methicillin-resistant Staphylococcus aureus at a tertiary care hospital in Japan. (2015)
  • Author(s): Inomata S, Yano H, Tokuda K, Kanamori H, Endo S, Ishizawa C, Ogawa M, Ichimura S, Shimojima M, Kakuta R, Ozawa D, Aoyagi T, Gu Y, Hatta M, Oshima K, Nakashima K, Kaku M.
  • Journal Title: J Infect Chemother. J Infect Chemother. Volume: 21 Issue: 10 Pages: 729-36
  • DOI: 10.1016/j.jiac.2015.07.005
  • Peer Reviewed
• [Journal Article] Minimally Invasive Treatment for Hard Palate-Invading Maxillary Keratocystic Odontogenic Tumor. (2015)
  • Author(s): Nomura K, Arakawa K, Fujishima F, Yamazaki Y, Ozawa D, Nomura Y, Hidaka H, Yoshida N, Katori Y.
  • Journal Title: Tohoku J Exp Med. Volume: 237 Issue: 4 Pages: 267-72
  • DOI: 10.1620/tjem.237.267
  • NAID: 130005108586
  • Peer Reviewed
• [Journal Article] Detection of Chlamydia trachomatis or Neisseria gonorrhoeae in otorhinolaryngology patients with pharyngeal symptoms (2014)
  • Author(s): Oda K, Yano H, Okitsu N, Chiba T, Hara Y, Kudo T, Ozawa D, Irimada M, and Ohyama K
  • Journal Title: Sexually Transmitted Infections Volume: 90 Issue: 2 Pages: 99-99
  • DOI: 10.1136/sextrans-2013-051419
  • Peer Reviewed
• [Journal Article] Severe acute otitis media caused by mucoid streptococcus pyogenes in a previously healthy adult. (2014)
  • Author(s): Kakuta R, Yano H, Hidaka H, Miyazaki H, Irimada M, Oda K, Arai K, Ozawa D, Takahashi T, Kaku M, Katori Y
  • Journal Title: Tohoku J Exp Med. Volume: 232 Pages: 301-304
  • NAID: 130004460123
  • Peer Reviewed
• [Journal Article] Twelve-year survey (2001-2012) of the antimicrobial susceptibility of Streptococcus pneumoniae isolates from otorhinolaryngology clinics in Miyagi Prefecture, Japan (2014)
  • Author(s): Ozawa D, Yano H, Hidaka H, Kakuta R, Komatsu M, Endo S, Kanamori H, Kaku M, and Katori Y
  • Journal Title: J Infect Chemother Volume: 20 Issue: 11 Pages: 702-708
  • DOI: 10.1016/j.jiac.2014.07.014
  • Peer Reviewed
• [Journal Article] Endoscopic drainage of orbital subperiosteal hematoma secondary to acute rhinosinusitis in a child. (2014)
  • Author(s): Nomura K, Honkura Y, Ozawa D, Okumura Y, Hidaka H, Ogawa T, Yamauchi D, Watanabe K, Katori Y
  • Journal Title: Tohoku J Exp Med. Volume: 233 Pages: 171-174
  • NAID: 130004460136
  • Peer Reviewed
• [Journal Article] Helicobacter cinaedi causing infected abdominal aortic aneurysm (2014)
  • Author(s): Kakuta R, Yano H, Kanamori H, Shimizu T, Gu Y, Hatta M, Aoyagi T, Endo S, Inomata S, Oe C, Tokuda K, Ozawa D, Goto H, Katori Y, and Kaku M
  • Journal Title: Emerg Infect Dis Volume: 20 Issue: 11 Pages: 1942-1945
  • DOI: 10.3201/eid2011.140440
  • Peer Reviewed
• [Presentation] 7価結合型肺炎球菌ワクチン導入後に本邦の小児急性中耳炎症例から分離された肺炎球菌の分子疫学的解析 (2016)
  • Author(s): 小澤大樹、矢野寿一、日高浩史、沖津尚弘、角田梨紗子、香取幸夫
  • Organizer: 第117回日本耳鼻咽喉科学会学術講演会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2016-05-18
• [Presentation] 7価結合型肺炎球菌ワクチン導入後に本邦の小児急性中耳炎症例から分離された肺炎球菌の分子疫学的解析 (2016)
  • Author(s): 6. 小澤大樹、矢野寿一、遠藤史郎、角田梨紗子、猪股真也、鈴木由希、斎藤恭一、藤川祐子、馬場啓聡、賀来満夫
  • Organizer: 第90回日本感染症学会学術講演会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-04-15