effect of antibiotics against biofilm formation of H.influenzae

Research Project

Project/Area Number	26462565
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Otorhinolaryngology
Research Institution	Wakayama Medical University
Principal Investigator	Takei Shin 和歌山県立医科大学, 医学部, 博士研究員 (40347589)
Co-Investigator(Kenkyū-buntansha)	土橋重貴和歌山県立医科大学, 医学部, 助教 (40725553) 戸川彰久和歌山県立医科大学, 医学部, 講師 (70305762) 山内一真和歌山県立医科大学, 医学部, 講師 (80336891)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000) Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	インフルエンザ菌 / バイオフィルム / 急性中耳炎 / 抗菌薬 / クラリスロマイシン
Outline of Final Research Achievements	Haemophilus influenzae(HI)is one of major causative pathogens of acute otitis media(AOM)and biofilm formation of nontypeable HI cause intractable AOM. We ivestigated effect of antibiotics against HI in vitro. amoxicilin(AMPC) did not show bacteriocidal effect at concentration over MIC but Clarithromycin(CAM) showed anti biofilm effect . We also investigated effect of concurrent therapy of CAM and AMPC against moderate AOM patients. group A is treated with AMPC, and group B was treated AMPC and CAM concurrently. group B showed significantly better effect with judgement of ENT clinicians.