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Analysis of the functional roles of TRPM4 in the mammalian cochlea

Research Project

Project/Area Number 26462568
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Otorhinolaryngology
Research InstitutionOsaka University (2015-2016)
Juntendo University (2014)

Principal Investigator

Murata Junko  大阪大学, 医学系研究科, 招へい准教授 (80332740)

Co-Investigator(Kenkyū-buntansha) 久恒 智博  国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (10321803)
Co-Investigator(Renkei-kenkyūsha) Okada Hiroko  順天堂大学, 医学部 耳鼻咽喉科学講座, 助教 (20433774)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsTRPM4 / 内耳発生 / 内有毛細胞 / 血管条辺縁細胞 / 聴性脳幹反応 / TRPチャンネル / 蝸牛 / 血管条
Outline of Final Research Achievements

We showed that in the cochleae of adult mice, the specific TRPM4-immuoreactivity was observed in the basolateral side of inner hair cells and in the apical side of marginal cells of stria vascularis by using the rabbit anti-TRPM4 polyclonal antibody. Additionally, the mRNA of TRPM4 was up-regulated both in the organ of Corti and in the stria vascularis at the stage when the auditory sense of mice is established. The examination using the auditory brain stem response (ABR) revealed that the hearing threshold was elevated in the TRPM4 knock-out mice compared to the wild type especially at the stage older than 17 weeks old. The spatial-temporal expression pattern of TRPM4 implicated that TRPM4, at least partially, may contribute the repolarization of inner hair cells and the transfer of potassium ion through the marginal cells to the endolymphatic space. The results of ABR suggest the TRPM4 gene may be related to the progressive hearing loss of the human.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results)

  • [Int'l Joint Research] Heidelberg University Medical Faculty(ドイツ)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Spatio-temporal expression of TRPM4 in the mouse cochlea2014

    • Author(s)
      Sakuraba M, Murata J (Corresponding author), Teruyama R, Kamiya K, Yamaguchi J, Okano H, Uchiyama Y
    • Journal Title

      Ikeda K J Neurosci Res

      Volume: (Epub ahead of print) Issue: 10 Pages: 1409-1418

    • DOI

      10.1002/jnr.23410

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] アスピリン喘息に対するアスピリン減感作療法とその鼻症状に対する改善効果について2014

    • Author(s)
      村田 潤子、熱田 了、八尾 隆史、高橋 和久、池田 勝久
    • Journal Title

      耳鼻咽喉科臨床

      Volume: 107 Pages: 948-949

    • NAID

      130004707193

    • Related Report
      2014 Research-status Report
  • [Presentation] TRPM4の内耳発生における発現とノックアウトマウスにみられた聴覚障害について2015

    • Author(s)
      村田 潤子、藤岡 正人、神崎 晶、岡田 弘子、池田 勝久、 小川 郁
    • Organizer
      第25回日本耳科学総会・学術講演会
    • Place of Presentation
      長崎ブリックホール(長崎市)
    • Year and Date
      2015-10-07
    • Related Report
      2015 Research-status Report
  • [Presentation] IP3 受容体 Type 3の内耳における発現とKOマウスを用いた聴覚系における機能解析の試み2014

    • Author(s)
      村田 潤子、城所 淑信、岡田 弘子、池田 勝久
    • Organizer
      第24回日本耳科学会・学術講演会
    • Place of Presentation
      朱鷺メッセ、新潟
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 哺乳類内耳発生における癌抑制遺伝子PTENの役割の多様性について2014

    • Author(s)
      村田 潤子、藤岡正人、神崎 晶、小川 郁、池田 勝久
    • Organizer
      第116回日本耳鼻咽喉科学会 総会・学術講演会
    • Place of Presentation
      東京国際フォーラム、東京
    • Year and Date
      2014-05-20 – 2014-05-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 哺乳類内耳おけるTRPM4の時空間的発現パターンが示唆するその機能について2014

    • Author(s)
      村田 潤子、城所 淑信、松岡理奈、神谷 和作、池田 勝久
    • Organizer
      第115回日本耳鼻咽喉科学会総会・学術講演会
    • Place of Presentation
      ヒルトン福岡シーホーク、福岡
    • Year and Date
      2014-05-14 – 2014-05-17
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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