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Clinical study using Autologous human nasal epithelial cell sheet for transplantation after middle ear surgery.

Research Project

Project/Area Number 26462569
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Otorhinolaryngology
Research InstitutionSt. Marianna University School of Medicine

Principal Investigator

Yuichiro Yaguchi  聖マリアンナ医科大学, 医学部, 准教授 (30307475)

Co-Investigator(Kenkyū-buntansha) 和田 弘太  東邦大学, 医学部, 教授 (20307482)
Research Collaborator Yamamoto Kazuhisa  
Morino Tunetaro  
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords再生医療 / 鼓室形成術 / 真珠腫性中耳炎 / 癒着性中耳炎 / 鼻粘膜上皮細胞シート / 中耳粘膜 / 細胞シート
Outline of Final Research Achievements

We developed a novel treatment method combining tympanoplasty and autologous nasal mucosal epithelial cell sheet transplantation for postoperative regeneration of the middle ear mucosa. In clinical research, we endoscopically removed an approximately 10×10mm piece of nasal mucosal tissue. Tissue-engineered autologous nasal mucosal epithelial cell sheets were fabricated by culturing the harvested cells in an aseptic environment in a good manufacturing practice-compliant cell processing facility. The cultivated cell sheets were transplanted, during tympanoplasty, onto the exposed bony surface of the attic of the tympanic and mastoid cavities where the mucosa had been lost. We performed this procedure on five patients with middle ear cholesteatoma and one patient with adhesive otitis media. All patients showed favorable postoperative course with no adverse events or complications and the patients’ hearing ability post-transplantation remained good.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Middle ear mucosal regeneration by tissue-engineered cell sheet transplantation.2017

    • Author(s)
      Yamamoto K, Yamato M, Morino T, Sugiyama H, Takagi R, Yaguchi Y, Okano T, Kojima H
    • Journal Title

      npj Regenerative Medicine

      Volume: 2 Issue: 1 Pages: 1-11

    • DOI

      10.1038/s41536-017-0010-7

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autologous human nasal epithelial cell sheet using temperature-responsive culture insert for transplantation after middle ear surgery.2017

    • Author(s)
      Hama T, Yamamoto K, Yaguchi Y, Murakami D, Sasaki H, Yamato M, Okano T, Kojima H.
    • Journal Title

      Journal Tissue Engineering Regenetive Medicine

      Volume: 10 Pages: 1089-1096

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Middle ear mucosal regeneration with three-dimensionally tissue-engineered autologous middle ear cell sheets in rabbit model.2016

    • Author(s)
      Yaguchi Y, Murakami D, Yamato M, Hama T, Yamamoto K, Kojima H, Moriyama H, Okano T.
    • Journal Title

      Journal of Tissue Engineering and Regenerative Medicine

      Volume: 10 Issue: 3 Pages: 188-194

    • DOI

      10.1002/term.1790

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 家兎モデルを用いた培養鼻粘膜細胞シート移植後における中耳粘膜再生の検討2017

    • Author(s)
      森野 常太郎, 山本 和央, 谷口 雄一郎, 小島 博己
    • Organizer
      日本耳科学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 培養上皮細胞シート移植による中耳粘膜再生治療の実現2017

    • Author(s)
      山本 和央, 森野 常太郎, 谷口 雄一郎, 小島 博己
    • Organizer
      日本耳鼻咽喉科学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The effect of transplantation of tissue-engineered cell sheets after middle ear surgery in a rabid model2016

    • Author(s)
      谷口 雄一郎
    • Organizer
      10th international conference on cholesteatoma and ear surgery
    • Place of Presentation
      Edinburgh(イギリス)
    • Year and Date
      2016-06-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Middle Ear Mucosal Regeneration with Tissue-Engineered Autologous Cell Sheets2015

    • Author(s)
      Yuichiro Yaguchi
    • Organizer
      BIT's 8th World Congress of Regenerative Medicine & Stem Cell
    • Place of Presentation
      Shahghai, China
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2014-04-04   Modified: 2019-03-29  

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