Effects of estrogen on the selective molecular transport in the inner ear

Research Project

Project/Area Number	26462570
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Otorhinolaryngology
Research Institution	Toho University
Principal Investigator	SUZUKI Mitsuya 東邦大学, 医学部, 教授 (50302724)
Co-Investigator(Kenkyū-buntansha)	岩崎真一東京大学, 医学部附属病院, 准教授 (10359606) 上羽瑠美東京大学, 医学部附属病院, 助教 (10597131) 坂本幸士東京大学, 医学部附属病院, 講師 (50323548) 牛尾宗貴東京大学, 医学部附属病院, 臨床登録医 (70361483)
Project Period (FY)	2014-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	ICRマウス / estrogen / ABR / 血液内耳関門 / Estrogen receptor　alpha / Estrogen receptor　beta / 卵巣摘出 / 蝸牛血液-内耳関門 / Estrogen receptor / エストロゲン / 血管条毛細血管 / 更年期 / 蝸牛外側壁 / 免疫染色 / 透過電顕 / 基底膜 / 陰性荷電 / エストロゲンα / エストロゲンβ / 蝸牛 / C57BL/6マウス
Outline of Final Research Achievements	As compared to that in the non-ovariectomized group (control) the ABR threshold in the ovariectomized group was significantly higher at 32 KHz at 1 month after surgery (3-months-old), and at 2, 4, 8, 16, and 32 kHz at 2 months after surgery (4-months-old). 2) The three-layer structure which consists of laminae rarae eterna and interna and lamina densa, could be not identified clearly in the capillary basement membrane in the stria vascularis at both 3-months-old and 4-months-old in both the ovariectomized group and non-ovariectomized group. Distribution of cationic polyethyleneimine (PEI) particles was hardly observed on the capillary basement membranes in the stria vascularis in both the ovariectomized group and control group. 3) No marked difference in expression of ER alpha and ER beta was observed in the cochlea between the control group and the ovariectomized group.