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Mechanisms of delayed degeneration of hair cells and spiral ganglion cell

Research Project

Project/Area Number 26462572
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Otorhinolaryngology
Research InstitutionNational Defense Medical College (2015-2016)
独立行政法人国立病院機構(東京医療センター臨床研究センター) (2014)

Principal Investigator

Mizutari Kunio  防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, 病院 耳鼻咽喉科科, 講師 (40338140)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords有毛細胞 / らせん神経節 / 遅発性細胞脱落 / 内耳エネルギー不全 / 蝸牛内電位 / 内耳 / エネルギー不全 / 3-nitropropionic acid / ミトコンドリア / 内有毛細胞 / 外有毛細胞 / 細胞死
Outline of Final Research Achievements

In this research, we analyzed the effect of low endocochlear potential on the survival of hair cells and spiral ganglion cells. First, we successfully made a mouse model of acute mitochondrial dysfunction used by 3-nitropropionic acid (3-NP). This mouse model had severe hearing loss measured by auditory brainstem response (ABR), which was caused by decrease of endocochlear potential. The gradient loss of hair cells and spiral ganglion cells was observed 2 weeks after 3-NP treatment, following severe degeneration of these cells, which causes permanent hearing impairment, was occurred. This degenerative changes were progressive, and progressed from the basal turn toward apex of the cochlea after 3-NP treatment.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 2014

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Acknowledgement Compliant: 2 results,  Open Access: 3 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Recovery of endocochlear potential after severe damage to lateral wall fibrocytes following acute cochlear energy failure2016

    • Author(s)
      Kitao, K. Mizutari, K. Nakagawa, S. Matsunaga, T. Fukuda, S. Fujii, M.
    • Journal Title

      Neuroreport

      Volume: 27 Issue: 15 Pages: 1159-66

    • DOI

      10.1097/wnr.0000000000000673

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Hyaluronic acid pretreatment for Sendai virus-mediated cochlear gene transfer.2016

    • Author(s)
      Kurioka T, Mizutari K, Niwa K, Fukumori T, Inoue M, Hasegawa M, Shiotani A.
    • Journal Title

      Gene Ther

      Volume: 23 Issue: 2 Pages: 187-95

    • DOI

      10.1038/gt.2015.94

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] NRF2 Is a Key Target for Prevention of Noise-Induced Hearing Loss by Reducing Oxidative Damage of Cochlea2016

    • Author(s)
      Honkura, Y. Matsuo, H. Murakami, S. Sakiyama, M. Mizutari, K. Shiotani, A. Yamamoto, M. Morita, I. Shinomiya, N. Kawase, T. Katori, Y. Motohashi, H.
    • Journal Title

      Sci Rep

      Volume: 6 Issue: 1 Pages: 19329-19329

    • DOI

      10.1038/srep19329

    • NAID

      130005260938

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High prevalence of CDH23 mutation in patients with congenital high-frequency recessive or sporadic hereditary hearing loss2015

    • Author(s)
      Mizutari K, Mutai H., Namba K., Miyanaga Y., Nakano A., Arimoto Y., Masuda S., Morimoto N., Sakamoto H., Kaga K., Matsunaga T.
    • Journal Title

      Orphanet Journal of Rare Diseases

      Volume: 10 Issue: 1 Pages: 60-60

    • DOI

      10.1186/s13023-015-0276-z

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spontaneous recovery of cochlear fibrocytes after severe degeneration caused by acute energy failure.2014

    • Author(s)
      Mizutari K
    • Journal Title

      Front Pharmacol.

      Volume: 5 Pages: 198-198

    • DOI

      10.3389/fphar.2014.00198

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Recovery of endocochlear potential after severe damage to lateral wall fibrocytes following acute cochlear energy failure2016

    • Author(s)
      Kunio Mizutari, Kyoko Kitao, Susumu Nakagawa, Tatsuo Matsunaga, Satoshi Fukuda, Masato Fujii
    • Organizer
      53rd Inner Ear Biology Workshop
    • Place of Presentation
      Montpellier, France
    • Year and Date
      2016-09-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2014-04-04   Modified: 2018-03-22  

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