| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
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| Outline of Final Research Achievements |
We examined whether olfactory sensory deprivation affected the dynamic incorporation of newly generated OSNs 3, 7, 14, and 28 days post-injury in adult mice. No significant differences in the numbers of olfactory marker protein (OMP)-positive mature OSNs or apoptotic OSNs were observed between the deprived and non-deprived sides 0-7 days post-injury. However, between days 7 and 28, the sensory deprived side showed markedly fewer OSNs and mature OSNs but more apoptotic OSNs than the non-deprived side. Intrinsic functional imaging of the dorsal surface of the olfactory bulb at day 28 revealed that responses to odor stimulation were weaker in the deprived side compared with those in the non-deprived side. These results indicate that in the adult OE sensory deprivation disrupts compensatory OSN regeneration following injury and that newly generated OSNs have a critical time window for sensory input-dependent survival 7-14 days post-injury.
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