Tumor necrosis factor-alpha antagonist suppresses local inflammatory reaction and facilitates olfactory nerve recovery following injury
| Project/Area Number |
26462579
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Mie University |
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Principal Investigator |
Miyamura Tomotaka 三重大学, 医学系研究科, リサーチアソシエイト (20581838)
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| Co-Investigator(Kenkyū-buntansha) |
小林 正佳 三重大学, 医学系研究科, 准教授 (80343218)
玉利 健悟 三重大学, 教養教育機構, 特任講師(教育担当) (90585176)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 嗅覚 / 鼻科学 / 再生医学 |
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| Outline of Final Research Achievements |
We investigated if TNF-alpha antagonist, etanercept is useful for functional recovery in the olfactory system following injury. We made a model of severe injury by performing olfactory nerve transection using a rigid stainless steel blade in transgenic (OMP-tau-lacZ) mice. Etanercept was injected intraperitoneally just after the nerve transection. Histological assessment of recovery within the olfactory bulb was made at 5, 14, 42 and 70 days after injury. X-gal staining was used to label the degenerating and regenerating olfactory nerve fibers and immunohistochemical staining to detect the presence of reactive astrocytes and macrophages. Etanercept-injected animals showed significant smaller areas of injury-associated tissue, less astrocytes and macrophages, and an increase in regenerating nerve fibers in a dose-dependent manner. These findings suggest that TNF-alpha antagonist can be useful as a therapeutic drug for olfactory dysfunction by head injury.
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Report
(5 results)
Research Products
(7 results)
