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Monophosphoryl lipid A enhances nontypeable Haemophilus influenzae-specific mucosal and systemic immune responses by intranasal immunization

Research Project

Project/Area Number 26462585
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Otorhinolaryngology
Research InstitutionOita University

Principal Investigator

SUZUKI MASASHI  大分大学, 医学部, 教授 (60211314)

Co-Investigator(Kenkyū-buntansha) 門脇 嘉宣  大分大学, 医学部, 助教 (10706980)
平野 隆  大分大学, 医学部, 講師 (20305056)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords中耳炎ワクチン / インフルエンザ菌 / 粘膜アジュバント
Outline of Final Research Achievements

We used monophosphoryl lipid A (MPL), a toll-like receptor (TLR) 4 agonist, as an adjuvant to induce mucosal immune responses against Nontypeable Haemophilus influenzae (NTHi)to enhance bacterial clearance from the nasopharynx. Mice were administered outer membrane protein (OMP) and 0, 10, or 20 micro g MPL intranasally. Control mice were administered phosphate-buffered saline. After immunization, these mice were challenged with NTHi,and then,the mice were killed and nasal washes were collected. The numbers of NTHi were counted and OMP-specific antibodies were quantified by enzyme-linked immunosorbent assay. The MPL 10 and 20 micro g group produced a significant reduction in the number of bacteria recovered from the nasal washes compared to the control group. OMP-specific IgA titers in nasal washes were also augmented in the MPL groups compared to the control and OMP groups. MPL is suitable for eliciting effective mucosal immune responses against NTHi in the nasopharynx.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2017 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Monophosphory lipid A enhances nontypeable Haemophilus influenzae-specific mucosal and systemic immune responses by intranasal immunization2017

    • Author(s)
      Taro Iwasaki,Takashi Hirano,Satoru Kodama,Yoshinori Kadowaki,Munehito Moriyama,Toshiaki Kawano,Masashi Suzuki
    • Journal Title

      International Journal of Pediatric Otorhinolaryngology

      Volume: 97 Pages: 5-12

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Monophosphoryl lipid A enhances nontypedable hHaemophilus influenzae-specific mucosal immune responses in the nasal mucosa2015

    • Author(s)
      Suzuki M., Hirano T., Iwasaki T., Kodama S., Moriyama Mu., Kadowaki Y., Kawano T
    • Organizer
      The 18th International Symposium on Recent Advances in Otitis Media,
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2015-06-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2014-04-04   Modified: 2018-03-22  

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