| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In this study, we newly constructed the RNA sequencing based miRNA expression signature by using clinical specimens. Based on the expression signature, we have identified several antitumor miRNAs and these miRNAs regulated oncogenic targets in head and neck squamous cell carcinoma (HNSCC) cells. miR-1 and miR-206 were significantly reduced HNSCC tissues and ectopic expression of these miRNAs inhibited HNSCC cancer cell aggressiveness. We found that two growth factor receptors, epidermal growth factor receptor (EGFR) and hepatocyte growth factor receptor (c-MET), were directly regulated by both miR-1 and miR-206 in HNSCC cells. Moreover, several reports showed that EGFR inhibitors induced c-MET signal activation, suggesting a possible mechanism of acquired resistance to EGFR inhibitors. The identification of targets modulated by tumor-suppressive miR-1 and miR-206 may lead to a better understanding of molecular pathogenesis of HNSCC.
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