Therapeutic approach to recurrent respiratory papillomatosis from the perspective of signal transduction of human papillomavirus

• Project/Area Number: 26462598
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Fukushima Medical University (2016); Kanazawa University (2014-2015)
• Principal Investigator: Murono Shigeyuki 福島県立医科大学, 医学部, 教授 (20345622)
• Co-Investigator(Renkei-kenkyūsha): YOSHIZAKI Tomokazu 金沢大学, 医学系, 教授 (70262582) ; WAKISAKA Naohiro 金沢大学, 医学系, 准教授 (70377414) ; KONDO Satoru 金沢大学, 大学病院, 講師 (70436822)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 喉頭乳頭腫 / HPV / VEGF / 血管新生 / うがい液

Outline of Final Research Achievements

Recurrent respiratory papillomatosis (RRP) is a refractory human papillomavirus (HPV)-associated disease. We tried to establish a tissue culture model of RRP. We confirmed growth of tumor cells in the primary tissue culture. However, continuing growth of those cells could not be achieved in following passage. On the other hand, immunohistochmestry was also performed to investigate a factor for a novel therapy. Expression of vascular endothelial growth factor (VEGF) was observed around fibrovascular cores of RRP. VEGF was observed in cores without an obvious vascular structure. Therefore, anti-VEGF antibody therapy can be a candidate for a novel therapy of RRP.

Research Products

• [Journal Article] Usefulness of human papillomavirus detection in oral rinse as a biomarker of oropharyngeal cancer (2017)
  • Author(s): Yoshida H, Murono S, Ueno T, Nakanishi Y, Tsuji A, Hatano M, Endo K, Kondo S, Sugimoto H, Wakisaka N, Yoshizaki T
  • Journal Title: Acta Otolaryngol Volume: - Issue: 7 Pages: 773-777
  • DOI: 10.1080/00016489.2016.1274426
  • Peer Reviewed
• [Journal Article] Intralesional cidofovir injection for recurrent respiratory papillomatosis in Japan (2016)
  • Author(s): Murono S, Nakanishi Y, Tsuji A, Endo K, Kondo S, Wakisaka N, Yoshizaki T
  • Journal Title: Auris Nasus Larynx Volume: 印刷中 Issue: 5 Pages: 541-545
  • DOI: 10.1016/j.anl.2016.01.005
  • Peer Reviewed
• [Journal Article] Trends in the management of recurrent respiratory papillomatosis in Japan (2015)
  • Author(s): Murono S, Nakanishi Y, Tsuji A, Endo K, Kondo S, Wakisaka N, Yoshida H, Yoshizaki T
  • Journal Title: Auris Nasus Larynx Volume: in press Issue: 3 Pages: 218-220
  • DOI: 10.1016/j.anl.2014.10.006
  • NAID: 120005516446
  • Peer Reviewed
• [Presentation] うがい液HPV検出の中咽頭癌バイオマーカーとしての可能性 (2016)
  • Author(s): 室野重之，上野貴雄，中西庸介，辻 亮，遠藤一平，近藤 悟，脇坂尚宏，吉崎智一
  • Organizer: 第40回日本頭頸部癌学会
  • Place of Presentation: ソニックシティ，さいたま市
  • Year and Date: 2016-06-09
• [Presentation] 喉頭乳頭腫におけるVEGF-Aの発現 (2016)
  • Author(s): 室野重之，中西庸介，辻 亮，遠藤一平，近藤 悟，脇坂尚宏，吉崎智一
  • Organizer: 第117回日本耳鼻咽喉科学会総会・学術講演会
  • Place of Presentation: 名古屋国際会議場，名古屋市
  • Year and Date: 2016-05-19
• [Presentation] Angiogenic factors in recurrent respiratory papillomatosis (2016)
  • Author(s): Murono S, Nakanishi Y, Yoshizaki T
  • Organizer: 19th World Congress for Bronchoesophagology
  • Place of Presentation: Fortezza da Basso, Florence, Italy
  • Year and Date: 2016-05-08
  • Int'l Joint Research
• [Presentation] Angiogenic factors in recurrent respiratory papillomatosis (2015)
  • Author(s): Shigeyuki Murono, Yosuke Nakanishi, Tomokazu Yoshizaki
  • Organizer: 30th International Papillomavirus Conference
  • Place of Presentation: Centro de Congressos de Lisboa, Lisbon, Portugal
  • Year and Date: 2015-09-17
  • Int'l Joint Research
• [Presentation] NBI内視鏡による喉頭乳頭腫の観察 (2014)
  • Author(s): 室野重之，吉崎智一
  • Organizer: 第66回日本気管食道科学会
  • Place of Presentation: 高知市 三翠園
  • Year and Date: 2014-11-13 – 2014-11-14