| Project/Area Number |
26462608
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Toho University (2016-2017)
Nagasaki University (2014-2015) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
福田 智美 東京慈恵会医科大学, 医学部, 講師 (40372776)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 口腔潰瘍 / 粘膜再生 / DNAメチル化 / Wnt5a / 再生医療 / 口腔咽頭科 |
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| Outline of Final Research Achievements |
Severe oral ulcers can lead to undernutrition and poor quality of life. In this study, we analyzed an oral ulcer model immunohistochemically using antibodies for the molecules as follows: PCNA, a marker for late G1 to S phase; 5-hydroxymethylcytosine (5-hmC), a DNA methylation marker; 5-methylcytosine (5-mC), a DNA demethylation marker; Dnmts, DNA methyltransferases; and Wnt5a, a regulator of epithelial stem/progenitor cell differentiation. As results, PCNA-positive cells increased at Day 2 and returned to normal at Day 6 after ulceration. The ratio of the expression level of 5-mC to 5-hmC declined at Day 5. Dnmts' expression pattern was compatible for these results. Moreover, Wnt5a-positive cells increased in the regenerating epithelium at Day 5. These results indicated that DNA methylation level was critically controlled in the process of oral mucosal regeneration and Wnt5a may possibly play an important role in regulation of stem/progenitor cell differentiation.
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