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Epigenetic regulation in the process of oral mucosal regeneration in a rat oral ulcer model

Research Project

Project/Area Number 26462608
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Otorhinolaryngology
Research InstitutionToho University (2016-2017)
Nagasaki University (2014-2015)

Principal Investigator

AKIYAMA Naotaro  東邦大学, 医学部, 助教 (90554238)

Co-Investigator(Kenkyū-buntansha) 福田 智美  東京慈恵会医科大学, 医学部, 講師 (40372776)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords口腔潰瘍 / 粘膜再生 / DNAメチル化 / Wnt5a / 再生医療 / 口腔咽頭科
Outline of Final Research Achievements

Severe oral ulcers can lead to undernutrition and poor quality of life. In this study, we analyzed an oral ulcer model immunohistochemically using antibodies for the molecules as follows: PCNA, a marker for late G1 to S phase; 5-hydroxymethylcytosine (5-hmC), a DNA methylation marker; 5-methylcytosine (5-mC), a DNA demethylation marker; Dnmts, DNA methyltransferases; and Wnt5a, a regulator of epithelial stem/progenitor cell differentiation. As results, PCNA-positive cells increased at Day 2 and returned to normal at Day 6 after ulceration. The ratio of the expression level of 5-mC to 5-hmC declined at Day 5. Dnmts' expression pattern was compatible for these results. Moreover, Wnt5a-positive cells increased in the regenerating epithelium at Day 5. These results indicated that DNA methylation level was critically controlled in the process of oral mucosal regeneration and Wnt5a may possibly play an important role in regulation of stem/progenitor cell differentiation.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2018 2017 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Keratinocyte Growth Factor (KGF) Modulates Epidermal Progenitor Cell Kinetics through Activation of p63 in Middle Ear Cholesteatoma2018

    • Author(s)
      Yamamoto-Fukuda Tomomi、Akiyama Naotaro、Takahashi Masahiro、Kojima Hiromi
    • Journal Title

      Journal of the Association for Research in Otolaryngology

      Volume: Epub ahead of print Issue: 3 Pages: 1-19

    • DOI

      10.1007/s10162-018-0662-z

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of YAP signaling in a rat tympanic membrane under a continuous negative pressure load and in human middle ear cholesteatoma.2017

    • Author(s)
      Akiyama N, Yamamoto-Fukuda T, Yoshikawa M, Kojima H.
    • Journal Title

      Acta Otolaryngol.

      Volume: 137 Issue: 11 Pages: 1158-1165

    • DOI

      10.1080/00016489.2017.1351040

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Evaluation of mechanotransduction in a rat tympanic membrane under a continuous negative pressure load and in human middle ear cholesteatoma.2018

    • Author(s)
      Akiyama N, Yamamoto-Fukuda T, Yoshikawa M, Kojima H
    • Organizer
      Association for Research in Otolaryngology The 41th Annual MidWinter Meeting
    • Related Report
      2017 Annual Research Report
  • [Presentation] Keratinocyte growth factor (KGF) modulates epidermal progenitor cell kinetics through activation of p63 in middle ear cholesteatoma.2018

    • Author(s)
      Yamamoto-Fukuda T, Akiyama N, Masahiro T, Kojima H
    • Organizer
      Association for Research in Otolaryngology The 41th Annual MidWinter Meeting
    • Related Report
      2017 Annual Research Report
  • [Presentation] Novel Experimental model for Negative Pressure in the Middle Ear and Effects of Epithelial-cell Proliferation in the Tympanic Membrane.2017

    • Author(s)
      Naotaro Akiyama, Tomomi Yamamoto-Fukuda, Haruo Takahashi and Hiromi Kojima
    • Organizer
      40th Annual MidWinter Meeting, Association for Research in Otolaryngology
    • Place of Presentation
      Baltimore, Maryland, USA
    • Year and Date
      2017-02-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ラット口腔潰瘍上皮化過程における Wnt5a 発現の解析2017

    • Author(s)
      穐山直太郎、福田 智美、吉川 衛
    • Organizer
      第118回日本耳鼻咽喉科学会通常総会・学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ラット中耳陰圧モデルおよび弛緩部型真珠腫における YAPシグナルの解析2017

    • Author(s)
      穐山直太郎、福田 智美、吉川 衛、小島 博己
    • Organizer
      第27回日本耳科学会総会・学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 中耳真珠腫発症機序の解明:中耳真珠腫形成における神経堤由来細胞の役割2017

    • Author(s)
      福田智美、穐山直太郎、高橋昌寛、小島博己
    • Organizer
      第27回日本耳科学会総会・学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ラット口腔潰瘍上皮化過程での粘膜上皮細胞における DNAメチルトランスフェラーゼ(Dnmt)発現の解析2016

    • Author(s)
      穐山直太郎、遠藤 大輔、小路 武彦
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      ビッグパレットふくしま(郡山市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] 真珠腫性中耳炎発生機序の分子形態学的解析2015

    • Author(s)
      福田 智美、小路 武彦
    • Organizer
      第47回日本臨床分子形態学会総会・学術集会
    • Place of Presentation
      長崎大学医学部良順会館・ポンぺ会館(長崎市)
    • Year and Date
      2015-09-18
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] KGF/FGF-7過剰発現モデルにおける上皮細胞増殖制御機構の解析2014

    • Author(s)
      穐山直太郎、福田智美、原川さゆみ、遠藤大輔、小路武彦
    • Organizer
      第46回日本臨床分子形態学会総会・学術集会
    • Place of Presentation
      TKP市ヶ谷カンファレンスセンター(東京都)
    • Year and Date
      2014-10-17
    • Related Report
      2014 Research-status Report
  • [Presentation] ペプチドハイドロゲル被包細胞移植によるラット中耳粘膜再生の検討2014

    • Author(s)
      穐山直太郎、福田智美、高橋晴雄、小路武彦
    • Organizer
      第55回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      松本市中央公民館(M ウイング文化センター)(長野県松本市)
    • Year and Date
      2014-09-27
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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