Research and development of the lymph node metastatic diagnosis using new molecular technique

Research Project

Project/Area Number	26462614
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Otorhinolaryngology
Research Institution	Fukushima Medical University
Principal Investigator	suzuki masahiro 福島県立医科大学, 医学部, 講師 (90513268)
Co-Investigator(Kenkyū-buntansha)	松塚崇福島県立医科大学, 医学部, 准教授 (80336461)
Project Period (FY)	2014-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	リンパ節転移 / OSNA法 / 頭頸部癌
Outline of Final Research Achievements	Background. This study aimed to investigate the effects of CK19 expression in the primary lesions of head and neck squamous cell carcinoma on the diagnosis of the cervical lymph node (CLN) metastasis using the one-step nucleic acid amplification (OSNA) assay.Methods. Primary lesions and 54 CLNs were resected from 21 patients with head and neck squamous cell carcinoma between 2009 and 2011. Each CLN was tested by the OSNA assay, and the CK19 mRNA copy number obtained was compared to the corresponding histopathological results.Results. In the primary lesion CK19-positive group, the sensitivity and specificity of the OSNA assay against hematoxylin-eosin staining were 86% and 100%,respectively. The p value by Fisher’s exact test was less than 0.0001, indicating statistical significance.Conclusion. These results suggest that OSNA offers similar diagnostic potential to that of histopathological diagnosis of CLN biopsy in patients with a CK19-positive primary lesion.