| Project/Area Number |
26462616
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Nagoya City University |
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Principal Investigator |
IJICHI Kei 名古屋市立大学, 大学院医学研究科, 助教 (50510278)
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| Co-Investigator(Kenkyū-buntansha) |
足立 誠 朝日大学, 歯学部, 講師 (10468192)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 唾液腺がん / 低酸素 / 悪性化 / HIF-1 / 耳下腺がん / 低酸素状態 / HIF-1α |
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| Outline of Final Research Achievements |
We conducted the study for the purpose of hypoxia environment elucidating a mechanism to promote the malignant transformation of the salivary gland cancer tissue.
Thyroid cancer cell line 8305c, TT and salivary gland cancer cell line A253 and NSDC-5F as a control were cultured in a hypoxia condition, then confirmed expression of HIF-1α in immunoblotting. HIF1α and β were expressed in A253 and NSDC-5F, TT, but was not able to confirm expression in 8305c. We observed a status of the epithelium mesenchyma transition and metastasis related protein in a hyponoxia condition similarly in immunoblotting, but there were no changes of the expression level in a hypoxia condition for TT and A253 where there was expression of HIF-1α.
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