| Project/Area Number |
26462621
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
高野澤 美奈子 自治医科大学, 医学部, 講師 (50316543)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 癌幹細胞 / 頭頸部癌 / CD44 / ALDH1A1 / CD133 / head and neck cancer |
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| Outline of Final Research Achievements |
We investigate about surface marker of head and neck cancer stem cell. At the first, we studied expression of CD44 or CD133 in five cell line (tongue squamous cell carcinoma). CD44richcell was detected in all cell lines. CD133richcell was detected in only one cell line, and the number of CD133richcell was small. Next step, we studied expression of CD44 or CD133 in two surgical resection samples. CD44richcell was many detected in one sample, but in the other sample CD44richcell was poorly detected. CD133richcell was few detected. So at further studies, we studied about expression of CD44v or ALDH1A1 as stem cell marker in four surgical resection samples. In two of four samples CD44vrichcell was detected, but in other two samples CD44vrichcell was poorly detected. ALDH1A1richcell was detected at about 20% of all sorted cells in all samples. Expression of ALDH1A1 was detected at stable rate. CD44, VD44v, and ALDH1A1 may be suitable marker of head and neck cancer stem cell.
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