| Project/Area Number |
26462623
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Keio University |
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Principal Investigator |
Ozawa Hiroyuki 慶應義塾大学, 医学部(信濃町), 講師 (30327621)
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| Co-Investigator(Renkei-kenkyūsha) |
WATANABE Yoshihiro 慶應義塾大学, 医学部, 助教 (30445374)
SATO Yoichiro 慶應義塾大学, 医学部, 助教 (40624440)
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| Research Collaborator |
Chung Christine Johns Hopkins University, Dept of Oncology Head and Neck therapeutic program
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 低酸素誘導因子 / 頭頸部癌 / 低酸素応答因子 / 転移 |
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| Outline of Final Research Achievements |
The aim of the present study was to investigate the molecular targets of HIF1-α relevant to metastasis, and to find out an impact of HIF1-α to HNSCC metastasis by clinicopathological assays. In immunohistochemical study, the HIF-1α expression was higher in cancer cells than in normal cells of the adjacent normal mucosa. Univariate analysis revealed that high expression of HIF-1α was significantly correlated with T-classification (P=0.012), lymph node metastasis (P=0.002) and lymphatic invasion (P=0.03). HIF-1 inhibitor downregulated the expression of HIF-1α, twist, Oct3/4 and Nanog of head and neck cancer cells.
These results suggest that HIF-1α plays a key role for the tumor progression of HNSCC through EMT and CSC property. From the present study, HIF-1α inhibition may have the possibility to suppress tumor metastasis, thus HIF-1α is attractive target for the treatment of HNSCC.
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