Establish the rapid diagnosis system for chemosensitivity on head and neck cancer treatment

• Project/Area Number: 26462625
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Aichi Medical University
• Principal Investigator: Ogawa Tetsuya 愛知医科大学, 医学部, 教授 (40334940)
• Co-Investigator(Kenkyū-buntansha): 吉川 和宏 愛知医科大学, 公私立大学の部局等, 特務教授 (60109759)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 抗がん薬感受性 / タンパク解析 / マイクロアレイ解析 / 機能解析 / 抗がん薬 / 頭頸部癌 / 頭頸部がん / 耐性化因子 / 網羅的解析 / マイクロアレイ

Outline of Final Research Achievements

Cisplation and 5FU are still mainstream chemotherapy drugs for head and neck carcinoma. We focused of protein and mRNA levels to gain higher precision result for identifying the chemoresistant factors.We used human HNSCC cell lines, cisplatin-sensitive, acquired cisplatin resistance, naturally cisplatin-resistant, and acquired 5-fluorouracil resistance. We performed proteomics analysis using iTRAQ and LC-ESI-MS/MS. We also did transcriptomics analysis using microarray. After integrating data, S100A2 a cisplatin-specific factor and BASP1 as multi-chemoresistance factor were identified. Functional analysis revealed expression S100A2 was reduced and recovered the cisplatin sensitivity.
This is a new frontier technique that will provide highly precise identifications as thinking of comprehensive analysis. Our findings indicate that these proteins can be used as criteria biomarkers for chemoresistance for head and neck cancer treatment.

Research Products

• [Presentation] Identification of chemoresistance factors for head and neck carcinoma by analysis with iTRAQ and Microarray (2018)
  • Author(s): Tetsuya Ogawa
  • Organizer: 17th Korea Japan Joint Meeting of Otorhinolaryngology head and neck surgery
  • Int'l Joint Research / Invited
• [Presentation] Identification of chemoresistance factors for head and neck carcinoma by analysis with iTRAQ and Micrarray (2015)
  • Author(s): Inukai D, Nishimura K, Okamoto T, Tsuchiya Y, Ijichi K, Ueda H, Ogawa T
  • Organizer: 13th japan-Taiwan Conference on Otolaryngology-Head and Neck Surgery
  • Place of Presentation: Hitotsubashi Hall, Tokyo
  • Year and Date: 2015-12-03
  • Int'l Joint Research
• [Presentation] 頭頸部癌細胞株を用いたiTRAQ法、マイクロアレイ法によるシスプラチン耐性化因子の網羅的解析 (2015)
  • Author(s): 犬飼大輔、小川徹也ら
  • Organizer: 第116回日本耳鼻咽喉科学会総会・学術講演会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2015-05-22