Analysis of oxidative stress in deep part of the glaucomatous eyes in mice by in vivo imaging using two photon excitation microscope

• Project/Area Number: 26462660
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Osaka University
• Principal Investigator: USUI SHINICHI 大阪大学, 医学系研究科, 講師 (20546882)
• Co-Investigator(Kenkyū-buntansha): 松下 賢治 大阪大学, 医学系研究科, 講師 (40437405)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ２光子励起顕微鏡 / 緑内障 / 酸化ストレス / 2光子顕微鏡 / 視神経 / アストロサイト / ２光子顕微鏡 / 2光子励起顕微鏡 / 網膜神経節細胞 / 神経保護

Outline of Final Research Achievements

We established an observation system in vivo for analyzing oxidative stress in mouse glaucoma model. For the analysis, the special fixed base for observing the deep part of the eyeball in mice under inhalation anesthesia using a two-photon-induced microscope was prepared. We also examined a method of exposing the optic nerve. Furthermore, an intravenous route of the tail vein was secured, and the drug could be administered at any time. The dosage and time course of the drug required to observe the target tissue in vivo using this system was examined in wild type mice.