A study relating to 4-dimentional imaging of aqueous outflow route and its dynamics in glaucoma pathology
| Project/Area Number |
26462664
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
谷原 秀信 熊本大学, 大学院生命科学研究部(医), 教授 (60217148)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 線維柱帯 / 緑内障 / 眼圧 / 房水 / イメージング / 房水流出路 / ライブイメージング / 貪食 / 線維柱帯細胞 / アクチン重合化 |
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| Outline of Final Research Achievements |
The aim of this study was to clarify the molecular mechanisms relating to the regulation of intraocular pressure in aqueous outflow tissue using novel imaging techniques. Observation of tissue around corneal limbus by two-photon microscopy depicted area which was supposed to be anterior chamber and Schlemm's canal. Collagen fibers in sclera were also imaged successfully. Despite of the consideration of imaging conditions, it was difficult to improve the signal/noise ratio of the images. In in vitro experiments, we could perceive the changes in actin cytoskeleton in trabecular meshwork cells after dexamethasone treatment, and firstly found decreased migration activity of the treated cells . Moreover, it was clarified that dexamethasone decreased phagocytic activity of trabecular meshwork cells, while ROCK inhibitor increased the activity.
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Report
(4 results)
Research Products
(33 results)
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[Presentation] 濾過胞内部の変化2014
Author(s)
第68回日本臨床眼科学会
Organizer
第68回日本臨床眼科学会
Place of Presentation
神奈川
Year and Date
2014-11-13 – 2014-11-16
Related Report
Invited
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