| Project/Area Number |
26462665
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
HIRAOKA MIKI 札幌医科大学, 医学部, 講師 (80246983)
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| Co-Investigator(Kenkyū-buntansha) |
大黒 浩 札幌医科大学, 医学部, 教授 (30203748)
吉田 香織 札幌医科大学, 医学部, 助教 (40596160)
阿部 晃 札幌医科大学, 医学部, 講師 (70136927)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 房水浄化機構 / 眼内炎症 / リン脂質 / リン脂質分解酵素 / 自己免疫性ぶどう膜炎 / 酸化リン脂質 / ぶどう膜炎 / リソゾームホスホリパーゼA２ / 自己免疫性 / リソゾーム / ホスホリパーゼA2 / ルイスラット / エンドトキシン |
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| Outline of Final Research Achievements |
The goal of this project is to clarify the involvement of phospholipids metabolism in ocular disease focusing the lysosomal phospholipase A2 (LPLA2).
In experimental autoimmune uveitis (EAU) model rats, the LPLA2 activity and the amount of phospholipids of the aqueous humor were concomitant with the extent of inflammation. The expression of LPLA2 and the production of oxidized phospholipid were observed in infiltrated macrophages. The LPLA2 deficient mice showed prolonged inflammation in endotoxin-induced uveitis. The clinical samples of aqueous humor from open-angle glaucoma patients showed higher LPLA2 activity than control group. In vitro, the hydrolysis of some truncated oxidized phospholipid (ox-PL) by LPLA2 was efficient compared to the non-oxidized phospholipids. Additionally, LPLA2 overexpressed cells catabolized truncated ox-PL and were protected from cell growth inhibition by ox-PL.
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