Exploratory study of novel neuroprotective treatment using microglia macrophages

• Project/Area Number: 26462677
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Tohoku University
• Principal Investigator: Maruyama Kazuichi 東北大学, 大学病院, 講師 (10433244)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: マイクログリア / マクロファージ / 緑内障 / NRF-2 / BDNF / ミクログリア

Outline of Final Research Achievements

The pathology of glaucoma is thought to be RGC death caused by obstruction of optic nerve axon flow to retinal ganglion cells (RGC). Various inflammatory or anti-inflammatory cytokines / chemokines are involved in the course of RGC death, and these cytokines / chemokines are produced from microglia, mueller cells or macrophages recruited into the retina after injury It is thought that. In this study, we observed microglia / macrophage kinetics at the time of disorder, and it turned out that cells associated with RGC death were macrophages and those with neuroprotective effect to suppress RGC death were microglia.

Research Products

• [Journal Article] Vitreous lavage fluid and bronchoalveolar lavage fluid have equal diagnostic value in sarcoidosis. (2016)
  • Author(s): Maruyama K, Inaba T, Tamada T, Nakazawa T.
  • Journal Title: Medicine (Baltimore) Volume: 95
  • Peer Reviewed / Open Access
• [Journal Article] Erratum: Estimation of Jones matrix, birefringence and entropy using Cloude-Pottier decomposition in polarization-sensitive optical coherence tomography: erratum. (2016)
  • Author(s): Yamanari M, Tsuda S, Kokubun T, Shiga Y, Omodaka K, Aizawa N, Yokoyama Y, Himori N, Kunimatsu-Sanuki S, Maruyama K, Kunikata H, Nakazawa T.
  • Journal Title: Biomed Opt Express Volume: 18 Pages: 46364638-46364638
  • Peer Reviewed
• [Journal Article] OCT-Based Quantification and Classification of Optic Disc Structure in Glaucoma Patients. (2016)
  • Author(s): Takada N, Omodaka K, Kikawa T, Takagi A, Matsumoto A, Yokoyama Y, Shiga Y, Maruyama K, Takahashi H, Akiba M, Nakazawa T.
  • Journal Title: PLoS One Volume: 24
  • Peer Reviewed / Open Access
• [Journal Article] Topical ocular dexamethasone decreases intraocular pressure and body weight in rats. (2016)
  • Author(s): Sato K, Nishiguchi KM, Maruyama K, Moritoh S, Fujita K, Ikuta Y, Kasai H, Nakazawa T.
  • Journal Title: J Negat Results Biomed. Volume: 12 Pages: 15-15
  • Peer Reviewed
• [Journal Article] The association between systemic oxidative stress and ocular blood flow in patients with normal-tension glaucoma. (2016)
  • Author(s): Himori N, Kunikata H, Shiga Y, Omodaka K, Maruyama K, Takahashi H, Nakazawa T.
  • Journal Title: Graefes Arch Clin Exp Ophthalmol. Volume: 254 Pages: 333341-333341
  • Peer Reviewed
• [Journal Article] Sufficient Evidence for Lymphatics in the Developing and Adult Human Choroid? (2015)
  • Author(s): Heindl LM, Kaser-Eichberger A, Schlereth SL, Bock F, Regenfuss B, Reitsamer HA, McMenamin P, Lutty GA, Maruyama K, Chen L, Dana R, Kerjaschki D, Alitalo K, De Stefano ME, Junghans BM, Schroedl F, Cursiefen C.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 56 Issue: 11 Pages: 6709-6710
  • DOI: 10.1167/iovs.15-17686
• [Journal Article] Stage-specific reference genes significant for quantitative PCR during mouse retinal development. (2015)
  • Author(s): Adachi, H., Tominaga, H., Maruyama, Y., Yoneda, K., Maruyama, K., Yoshii, K., Kinoshita, S., Nakano, M., Tashiro, K.
  • Journal Title: Genes Cells Volume: 20 Issue: 8 Pages: 625-635
  • DOI: 10.1111/gtc.12254
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular, anatomical and functional changes in the retinal ganglion cells after optic nerve crush in mice (2015)
  • Author(s): Yukita M, Machida S, Nishiguchi KM, Tsuda S, Yokoyama Y, Yasuda M, Maruyama K, Nakazawa T.
  • Journal Title: Doc Ophthalmol. Volume: 130 Issue: 2 Pages: 149-156
  • DOI: 10.1007/s10633-014-9478-2
  • Peer Reviewed / Open Access
• [Journal Article] Critical neuroprotective roles of heme oxygenase-1 induction against axonal injury-induced retinal ganglion cell death. (2014)
  • Author(s): 8.Himori N, Maruyama K, Yamamoto K, Yasuda M, Ryu M, Omodaka K, Shiga Y, Tanaka Y, Nakazawa T.
  • Journal Title: J Neurosci Res Volume: 9 Issue: 9 Pages: 1134-42
  • DOI: 10.1002/jnr.23398
  • Peer Reviewed / Open Access
• [Journal Article] Neuroprotective effect against axonal damage-induced retinal ganglion cell death in apolipoprotein E-deficient mice through the suppression of kainate receptor signaling. (2014)
  • Author(s): 10.Omodaka K, Nishiguchi KM, Yasuda M, Tanaka Y, Sato K, Nakamura O, Maruyama K, Nakazawa T.
  • Journal Title: Brain Res Volume: 1586 Pages: 203-212
  • DOI: 10.1016/j.brainres.2014.08.053
  • Peer Reviewed
• [Journal Article] The role of calpain in an in vivo model of oxidative stress-induced retinal ganglion cell damage (2014)
  • Author(s): Yu Yokoyama, Kazuichi Maruyama, Kotaro Yamamoto, Kazuko Omodaka, Masayuki Yasuda, Noriko Himori, Morin Ryu, Koji M. Nishiguchi, Toru Nakazawa
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 451 Issue: 4 Pages: 510-515
  • DOI: 10.1016/j.bbrc.2014.08.009
  • Peer Reviewed / Open Access
• [Journal Article] The effect of podoplanin inhibition on lymphangiogenesis under pathological conditions. (2014)
  • Author(s): 5.Maruyama Y, Maruyama K, Kato Y, Kajiya K, Moritoh S, Yamamoto K, Matsumoto Y, Sawane M, Kerjaschki D, Nakazawa T, Kinoshita S
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 55 Issue: 8 Pages: 4813-4822
  • DOI: 10.1167/iovs.13-13711
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 再発型Vogt-小柳-原田病におけるシクロスポリン有用性の検討 (2016)
  • Author(s): 丸山和一
  • Organizer: 第70回日本臨床眼科学会
  • Place of Presentation: 京都市 京都国際会議場
  • Year and Date: 2016-11-03
• [Presentation] 神経保護におけるマクロファージと ミクログリア（マイクログリア）の役割 (2016)
  • Author(s): 丸山 和一
  • Organizer: 第27回日本緑内障学会
  • Place of Presentation: 横浜市 パシフィコ横浜
  • Year and Date: 2016-09-17
  • Invited
• [Book] あたらしい眼科 (2016)
  • Author(s): 丸山 和一
  • Total Pages: 6
  • Publisher: メディカル葵出版
• [Book] 医学のあゆみ (2016)
  • Author(s): 丸山 和一
  • Total Pages: 3
  • Publisher: 医歯薬出版