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Development of Raman imaging technique of Mitochondorial function in optic neuropathies

Research Project

Project/Area Number 26462684
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ophthalmology
Research InstitutionOsaka University

Principal Investigator

Morimoto Takeshi  大阪大学, 医学系研究科, 准教授 (00530198)

Project Period (FY) 2014-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywordsラマン分光法 / 視神経疾患 / ミトコンドリア機能 / 酸化ストレス / 細胞死 / 網膜神経節細胞 / ミトコンドリア病 / cytochrome c / ミトコンドリア / ハイブリッドラマンイメージング / cytochrome c / ネクローシス / レーベル病 / Raman spectroscopy / Cell death / Oxidative stress / Neuroprotection
Outline of Final Research Achievements

Mitochondrial activity is a widely used criterion to judge the metabolic condition of a living specimen. In this study, we demonstrate that the redox state of cytochromes can serve as a sensitive mitochondrial activity indicator in glutamate-stressed neuronal cells. Mitochondrial dysfunction was detected by Raman imaging as early as 30 min after glutamate-stress induction. By comparing this result with other commonly used mitochondrial function assays, we found Ramanimaging has a similar sensitivity to ATP production and trans-membrane potential assays. Other viabilitytests, such as MTT assay and ROS production tests, showed a slower response than our method. A thorough understanding of cytochrome dynamics with our new method will help establish Raman spectroscopy as a competitive clinical diagnosis tool for neurodegenerative diseases involving mitochondrial dysfunction.

Academic Significance and Societal Importance of the Research Achievements

ラマン分光法を眼科領域に用いた研究は少なく、本研究は、網膜細胞の酸化還元状態をラマン分光法で評価できることを示した、最初の論文であり、学術的意義が高いと考える。また本研究によって、今後、ラマン分光法の眼科領域への応用が活発になされるものと考える。また、ラマン分光法は従来の分子生物学的検査とはことなり、物体に照射した光の反射のうちラマン散乱光を測定するだけであり、ラマン分光法を捉えるためには特殊な計測装置は必要であるため簡便ではないが、生体を固定したり、染色したりする必要がなく、生体のありのままの状態を捉えることができるため、将来の臨床応用が可能であり社会的な意義は大きい。

Report

(6 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (39 results)

All 2019 2018 2017 2016 2015 2014

All Journal Article (19 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 19 results,  Open Access: 17 results,  Acknowledgement Compliant: 3 results) Presentation (20 results) (of which Int'l Joint Research: 14 results,  Invited: 3 results)

  • [Journal Article] Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction.2019

    • Author(s)
      Morimoto T, Chiu LD, Kanda H, Kawagoe H, Ozawa T, Nakamura M, Nishida K, Fujita K, Fujikado T
    • Journal Title

      Analyst

      Volume: 144 Issue: 8 Pages: 25312540-25312540

    • DOI

      10.1039/c8an02213e

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Light localization with low-contrast targets in a patient implanted with a suprachoroidal?transretinal stimulation retinal prosthesis2018

    • Author(s)
      Endo Takao、Fujikado Takashi、Hirota Masakazu、Kanda Hiroyuki、Morimoto Takeshi、Nishida Kohji
    • Journal Title

      Graefe's Archive for Clinical and Experimental Ophthalmology

      Volume: printing Issue: 9 Pages: 1-7

    • DOI

      10.1007/s00417-018-3982-0

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Objective evaluation of visual fatigue using binocular fusion maintenance2018

    • Author(s)
      Hirota Masakazu、Morimoto Takeshi、Kanda Hiroyuki、Endo Takao、Miyoshi Tomomitsu、Miyagawa Suguru、Hirohara Yoko、Yamaguchi Tatsuo、Saika Makoto、Fujikado Takashi
    • Journal Title

      Translational Vision Science & Technology

      Volume: 7 Issue: 2 Pages: 9-9

    • DOI

      10.1167/tvst.7.2.9

    • URL

      https://pure.teikyo.jp/en/publications/4046667b-ce25-4c17-b748-53fb5c888269

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The relationship between retinal damage and current intensity in a pre-clinical suprachoroidal-transretinal stimulation model using a laser-formed microporous electrode2017

    • Author(s)
      Kanda Hiroyuki、Nakano Yukari、Terasawa Yasuo、Morimoto Takeshi、Fujikado Takashi
    • Journal Title

      Journal of Neural Engineering

      Volume: 14 Issue: 5 Pages: 056013-056013

    • DOI

      10.1088/1741-2552/aa7d5c

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Automated measurements of human cone photoreceptor density in healthy and degenerative retina by region-based segmentation2017

    • Author(s)
      Miyagawa Suguru、Fukuyama Hisashi、Hirota Masakazu、Yamaguchi Tatsuo、Kitamura Kazuo、Endo Takao、Kanda Hiroyuki、Morimoto Takeshi、Fujikado Takashi
    • Journal Title

      Clin Ophthalmol

      Volume: 11 Pages: 781

    • DOI

      10.2147/opth.s133070

    • URL

      https://localhost/en/publications/ebbc136d-8a1d-40f8-a2dd-ca1b01e717e2

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prevalence and Associated Factors of Segmentation Errors in the Peripapillary Retinal Nerve Fiber Layer and Macular Ganglion Cell Complex in Spectral-domain Optical Coherence Tomography Images2017

    • Author(s)
      Miki Atsuya、Kumoi Miho、Usui Shinichi、Endo Takao、Kawashima Rumi、Morimoto Takeshi、Matsushita Kenji、Fujikado Takashi、Nishida Kohji
    • Journal Title

      Journal of Glaucoma

      Volume: 26 Issue: 11 Pages: 995-1000

    • DOI

      10.1097/ijg.0000000000000771

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Relationships Between Spatial Contrast Sensitivity and Parafoveal Cone Density in Normal Subjects and Patients With Retinal Degeneration.2017

    • Author(s)
      Hirota M, Morimoto T, Kanda H, Lohmann TK, Miyagawa S, Endo T, Miyoshi T, Fujikado T.
    • Journal Title

      Ophthalmic Surg Lasers Imaging Retina.

      Volume: 48(2) Issue: 2 Pages: 106-113

    • DOI

      10.3928/23258160-20170130-03

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 独立成分分析を用いた4種類の刺激に対する網膜内因性信号の検討2016

    • Author(s)
      三橋俊文、広原陽子、神田寛行、大川賀孝、三好智満、森本壮、不二門尚
    • Journal Title

      視覚の科学

      Volume: 37 Pages: 7-17

    • NAID

      130006943344

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] One-year outcome of 49-channel suprachoroidal–transretinal stimulation prosthesis in patients with advanced retinitis pigmentosa2016

    • Author(s)
      Fujikado T, Kamei M, Sakaguchi H, Kanda H, Endo T, Hirota M, Morimoto T, Nishida K, Kishima H, Terasawa Y, Oosawa K, Ozawa M, Nishida K.
    • Journal Title

      Invest Ophthalmol Vis Sci.

      Volume: 57 Issue: 14 Pages: 6147

    • DOI

      10.1167/iovs.16-20367

    • URL

      https://pure.teikyo.jp/en/publications/0ed381db-22e6-48e2-af43-64cc308bcf3e

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Relationship between reading performance and saccadic disconjugacy in patients with convergence insufficiency type intermittent exotropia.2016

    • Author(s)
      Hirota M, Kanda H, Endo T, Lohmann TK, Miyoshi T, Morimoto T, Fujikado T.
    • Journal Title

      Jpn J Ophthalmol.

      Volume: 60(4) Issue: 4 Pages: 326-332

    • DOI

      10.1007/s10384-016-0444-2

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] False reaching movements in localization test and effect of auditory feedback in simulated ultra-low vision subjects and patients with retinitis pigmentosa2016

    • Author(s)
      Endo T, Kanda H, Hirota M, Morimoto T, Nishida K, Fujikado T.
    • Journal Title

      Graefes Arch Clin Exp Ophthalmol.

      Volume: 254 Issue: 5 Pages: 947

    • DOI

      10.1007/s00417-015-3253-2

    • URL

      https://pure.teikyo.jp/en/publications/cbf3ce2b-119c-46a1-8a8b-a90a27287674

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Stereoscopic perception of 3-D images by patients after surgery for esotropia.2016

    • Author(s)
      Endo T, Fujikado T,ShimojyoH, KandaH, Morimoto T, NishidaK
    • Journal Title

      Jpn J Ophthalmol

      Volume: 60 Issue: 1 Pages: 7-13

    • DOI

      10.1007/s10384-015-0419-8

    • NAID

      210000176393

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Surgical feasibility and biocompatibility of wide-field dual-array suprachoroidal-transretinal stimulation prosthesis in middle-sized animals.2016

    • Author(s)
      LohmanTK, Kanda H, Morimoto T, Endo T, MiyoshiT, Nishida K, Kamei M, Walter P, Fujikado T
    • Journal Title

      Graefes Arch Clin Exp Ophthalmol

      Volume: 254 Issue: 4 Pages: 661-673

    • DOI

      10.1007/s00417-015-3104-1

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Retinal nerve fiber layer and ganglion cell complex thicknesses measured with spectral-domain optical coherence tomography in eyes with no light perception due to nonglaucomatous optic neuropathy.2015

    • Author(s)
      Miki A, Endo T, Morimoto T, Matsushita K, Fujikado T, Nishida K
    • Journal Title

      Jpn J Ophthalmol

      Volume: 59 Issue: 4 Pages: 230-235

    • DOI

      10.1007/s10384-015-0386-0

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Slow cone reflectance changes during bleaching determined by adaptive optics scanning laser ophthalmoscope in living human eyes2015

    • Author(s)
      Hirota M, Miyagawa S, Kanda H, Endo T, Lohmann TK, Miyoshi T, Morimoto T, Fujikado T.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 6 Pages: e0131485-e0131485

    • DOI

      10.1371/journal.pone.0131485

    • URL

      https://pure.teikyo.jp/en/publications/c039f94b-00c9-43de-95b5-b0fa41889389

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluation of electrochemically treated bulk electrodes for a retinal prosthesis by examination of retinal intrinsic signals in cats2015

    • Author(s)
      Kanda H, Mihashi T, Miyoshi T, Hirohara Y, Morimoto T, Terasawa Y, Fujikado T.
    • Journal Title

      Jpn J Ophthalmol

      Volume: 58 Issue: 4 Pages: 309

    • DOI

      10.1007/s10384-014-0319-3

    • URL

      https://localhost/en/publications/b37efb91-6a7e-42d1-9acc-86ab95ad3a03

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Asymmetric wavefront aberrations and pupillary shapes induced by electrical stimulation of ciliary nerve in cats measured with compact wavefront aberrometer2015

    • Author(s)
      Miyagawa S, Mihashi T, Kanda H, Hirohara Y, Endo T, Morimoto T, Miyoshi T, Fujikado T
    • Journal Title

      PLoS One

      Volume: 9 Issue: 8 Pages: e105615-e105615

    • DOI

      10.1371/journal.pone.0105615

    • URL

      https://pure.teikyo.jp/en/publications/7f79f914-2bed-4923-b563-fa81767c6440

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characteristics of retinal reflectance changes induced by transcorneal electrical stimulation in cat eyes2014

    • Author(s)
      Morimoto Takeshi, Kanda H, Miyoshi T, Hirohara Y, Mihashi T, Kitaguchi Y, Nishida K, Fujikado T
    • Journal Title

      PLOS ONE

      Volume: 9 Issue: 3 Pages: 1-11

    • DOI

      10.1371/journal.pone.0092186

    • URL

      https://pure.teikyo.jp/en/publications/d02d3244-69ce-49d3-9994-eb7f7944bebe

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] National clinical investigation of anti-aquaporin-4 seropositive optic neuritis2014

    • Author(s)
      Endo T, Fujikado T, Morimoto T, Mimura O, Nishida K.
    • Journal Title

      Nihon Ganka Gakkai Zasshi.

      Volume: 118 Pages: 751-758

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] In situ monitoring the redox dynamics of cytochrome c in glutamate-induced dying retinal cells by hybrid fluorescence-Raman imaging2018

    • Author(s)
      Morimoto T, Chiu LD, Kanda H,, Nishida K, Fujita K, Fujikado T.
    • Organizer
      ARVO2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Binocular Coordination during Smartphone Reading in Patients with Intermittent Exotropia2018

    • Author(s)
      Hirota Masakazu、Kanda H、Endo T、Morimoto T、Miyoshi T、Fujikado T
    • Organizer
      ISA + AAPOS 2018
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development and evaluation of a wide-field dual-array suprachoroidal-transretinal stimulation(STS) prosthesisu system.2017

    • Author(s)
      Takeshi Morimoto、H Kanda、K Hozumi、K Nishida、T Fujikado
    • Organizer
      Artificial Vision 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Temporal ptterns of single-unit responses from on- and off-cells in the lateral geniculate nucleus elicited by suprachoroidal-transretinal stimulation.2017

    • Author(s)
      Hiroyuki Kanda、T Miyoshi 、T Morimoto、T Fujikado
    • Organizer
      Artificial Vision 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Outcome measure of clinical traial of retinal prosthesis by suprachoroidal-transretinal stimulation(STS) for patients with hand-motion vision.2017

    • Author(s)
      Takashi Fujikado、T Endo、K Hozumi、H Kanda、T Morimoto、M Hirota、M Ozawa
    • Organizer
      Artificial Vision 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Evaluation of reaching movement and mobility with a prosthetic vision simulator.2017

    • Author(s)
      Kenta Hozumi、T Endo、M Hirota、H Kanda、T Morimoto、K Nishida、T Fujikado
    • Organizer
      Artificial Vision 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of a Second-Generation Device for Suprachoroidal-Transretinal Stimulation2017

    • Author(s)
      Kanda H、Kamei M、Sakaguchi H、Endo T、Hirota M、Morimoto T、Nishida K、Kishima H、Miyoshi T、Terasawa Y、Osawa K、Ozawa M、Fujikado T
    • Organizer
      39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Relationship between reading performance and saccadic disconjugacy in patients with convergence insufficiency type intermittent exotropia.2017

    • Author(s)
      Hirota Masakazu、Kanda H、Endo T、T K Lohmann、Miyoshi T、Morimoto T、Fujikado T
    • Organizer
      第73回日本弱視斜視学会総会・第42回日本小児眼科学会総会合同学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Objective Assessment of Visual Fatigue by Ability to Maintain Binocular Fusion.2017

    • Author(s)
      Hirota M、Morimoto T、Kanda H、Endo T、Miyoshi T、Miyagawa S、Hirohara Y、Yamaguchi T、Saika M、Fujikado T
    • Organizer
      ARVO2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Chronic Implantation of a wide-field dual-array suprachoroidaltransretinal stimulation (STS) prosthesis in dogs.2017

    • Author(s)
      Morimoto T、Kanda H、Endo T、Nishida K、Fujikado T
    • Organizer
      ARVO2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Novel eye-tracking method for retinal prostheses.2017

    • Author(s)
      Kanda H、Satonaka S、Morimoto T、Miyoshi T、Fujikado T
    • Organizer
      ARVO2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Optimized position of dualarray STS retinal prosthesis for mobility examined by retinal prosthesis simulator.2017

    • Author(s)
      Hozumi K、Endo T、Hirota、Kanda H、Morimoto T、Fujikado T、Nishida K
    • Organizer
      ARVO2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ラマン顕微鏡を用いた網膜神経節細胞の細胞死の観察 ラマンイメージングの医科学への応用2017

    • Author(s)
      森本 壮、Liang-da Chiu、藤田克昌, 神田寛行, 不二門 尚
    • Organizer
      第9回フォトニクスシンポジウム フォトニクスとイノベーション
    • Place of Presentation
      大手町サンケイプラザ 4階ホール
    • Related Report
      2016 Research-status Report
  • [Presentation] Waching dying retinal ganglion cells induced by high concentration o f glutaminate by using Raman microscopy.2015

    • Author(s)
      Morimoto T, chiuLiang-da, Fujita K, Kanda H, FujikadoT
    • Organizer
      Nanophotonics in Asia 2015
    • Place of Presentation
      大阪 阪大中の島センター
    • Year and Date
      2015-12-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Surgical feasibility of wide-field dual-array suprachoroidal-transtretinal stimulation(STS) prosthesis in middle-sized animals.2015

    • Author(s)
      Morimoto T, Kanda H, Miyoshi T, Lohmann T.K, Fujikado T
    • Organizer
      Artifitial Vision 2015
    • Place of Presentation
      Center for Technology Aachen Europaplatz, Germany
    • Year and Date
      2015-11-28
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Current intensity-dependent neuroprotection in eyes with traumatic optic neuropathy py transcorneal electrical stimulation.2015

    • Author(s)
      Morimoto T, Endo T, Nishida K, Fujikado T
    • Organizer
      ARVO2015
    • Place of Presentation
      USA(Denver)
    • Year and Date
      2015-05-03
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Evaluation of inner retinal layers by phosphene and OCT in patients with advanced retinitis pigmentosa.2015

    • Author(s)
      Morimoto T
    • Organizer
      ASIA ARVO2015
    • Place of Presentation
      yokohama
    • Year and Date
      2015-02-16
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Watching dying RGC-5 cells by using Raman microscopy 22014

    • Author(s)
      Morimoto T, Kanda H, Endo T, Nishida K,Fujikado T.
    • Organizer
      ARVO2014
    • Place of Presentation
      Orlando USA
    • Year and Date
      2014-05-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 視神経炎に対する経角膜電気刺激治療2014

    • Author(s)
      森本 壮
    • Organizer
      第118回日本眼科学会総会
    • Place of Presentation
      東京
    • Year and Date
      2014-04-06
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Watching dying RGC-5 cells by using Raman microscopy2014

    • Author(s)
      Morimoto T, Kanda H, Endo T, Nishida K,Fujikado T.
    • Organizer
      WOC2014
    • Place of Presentation
      Tokyo
    • Year and Date
      2014-04-05
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2020-03-30  

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