Elucidation of the mechanism of epithelial stem cell niche and its reproduction
| Project/Area Number |
26462685
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Osaka University |
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Principal Investigator |
Hayashi Ryuhei 大阪大学, 医学系研究科, 寄附講座准教授 (70535278)
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| Research Collaborator |
YAMAMOTO Masayuki 東北大学, メディカル・メガバンク機構, 機構長
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 幹細胞ニッチ / 角膜上皮幹細胞 / N-cadherin / NRF2 / 角膜上皮 / Nrf2 / Ｎ－ｃａｄｈｅｒｉｎ / Ｎｒｆ２ / 幹細胞 |
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| Outline of Final Research Achievements |
In this study, we aimed to elucidate the epithelial stem cell niche using the corneal epithelial stem cells as a model of stem cell niche focusing on N-cadherin and NRF2, and developed a new corneal epithelial culture system-based on the information obtained there. As a result, we clarified a part of the mechanism for regulation of N-cadherin expression, and also showed that this mechanism also contributes to normal differentiation of corneal epithelium. From the results of this research, we successfully differentiated corneal epithelial (stem) cells from pluripotent stem cells. In addition, it was found that activation of NRF2 remarkably increases corneal epithelial stem cell survival activity. Moreover, based on the results of this research, we succeeded in development of conservation method for epithelial stem cells focusing on NRF2 activation mechanism.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Co-ordinated ocular development from hiPSCs and recovery of anterior eye function.2016
Author(s)
Hayashi R, Ishikawa Y, Sasamoto Y, Katori R, Nomura N, Ichikawa T, Araki S, Soma T, Kawasaki S, Sekiguchi K, Quantock A, Tsujikawa M, & Nishida K.
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Journal Title
Nature
Volume: 17;531(7594)
Issue: 7594
Pages: 376-80
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes.2016
Author(s)
Sasamoto Y, Hayashi R, Park S, Saito-Adachi M, Suzuki Y, Kawasaki S, Quantock A, Nakai K, Tsujikawa M, and Nishida K
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Journal Title
Scientific Reports
Volume: 22;6:20807
Issue: 1
Pages: 20807-20807
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Induction of Corneal Epithelial Cells from Human Pluripotent Stem Cells by a Method Based on Spontaneous Ocular Cell Differentiation2016
Author(s)
Ryuhei Hayashi, Yuki Ishikawa, Yuzuru Sasamoto, Ryosuke Katori, Tatsuya Ichikawa, Naoki Nomura, Takeshi Soma, Satoshi Kawasaki, Andrew J Quantock, Kiyotoshi Sekiguchi, Motokazu Tsujikawa, and Kohji Nishida
Organizer
ISSCR
Place of Presentation
米国サンフランシスコ
Related Report
Int'l Joint Research
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[Book] 腎と透析2016
Author(s)
林竜平、西田幸二
Total Pages
5
Publisher
株式会社 東京医学社
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