| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Since it is important to determine the mechanism of cell death independent of the intraocular pressure (IOP), we examined gene expression changes in the retina after the systemic administration of aldosterone. After the subcutaneous implantation of an osmotic minipump into the mid-scapular region of rats, we administered an 80 μg/kg/day dose of aldosterone.Analysis of the microarray data sets revealed the upregulation of 24 genes and the downregulation of 24 genes of key apoptosis-specific genes. Real-time PCR revealed 5 genes (Bcl3, Cdkn1a, Tbox5, Pf4, and Vdr) were upregulated while 10 genes (Asns, Bard1, Card9, Fcgr1a, Inhba, Kcnh8, Lck, Phlda1, Ptprc, and Sh3rf1) were downregulated. Significant increases or decreases were noted in several genes after the systemic administration of aldosterone. Further studies will need to be undertaken in order to definitively clarify the role of these genes in the eyes of animals with normal-tension glaucoma.
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