Bone Marrow Mesenchymal Cell Transplantation for thin Corneal Stroma

• Project/Area Number: 26462690
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Ehime University
• Principal Investigator: Yuichi Ohashi 愛媛大学, 本部, 学長 (00116005)
• Co-Investigator(Kenkyū-buntansha): 林 康人 愛媛大学, 医学部附属病院, 講師 (70314953)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ケラトサイト / バイオイメージング / 骨髄線維芽細胞 / 再生医療 / 細胞増殖 / Fucci / Creレポーターマウス / 骨髄細胞

Outline of Final Research Achievements

Bone Marrow Mesenchymal Cell Transplantation for thin Corneal Stroma
ROSA26 (CAG-loxP-NeoR-loxP- mVenus -hGem (1/110) IRES2 mCherry -hCdt1 (30/120) ) knock-in mouse lines were newly developed by gene targeting technique using C57BL/6-derived ES cells for tissue specific cell cycle bio-imaging. These mice were mated with Keratocan-IRES2-nls-Cre to get bone marrow mesenchymal cell culture (BMC) . The BMCs were injected into artificial thin cornea stroma. The transplanted BMC proliferation were observed a confocal microscope or a multi-photon microscope in-vivo or ex-vivo. Corneal stromal opacities were appeared two weeks after BMC injection. Over-proliferation of BMCs increase the thickness of corneal stroma, but ruin its clarity.

Research Products

• [Presentation] ケラトサイト細胞増殖周期のFucci2バイオイメージング (2016)
  • Author(s): 林康人
  • Organizer: 第120回 日本眼科学会総会
  • Place of Presentation: 仙台国際センター（宮城県、仙台市）
  • Year and Date: 2016-04-07
• [Presentation] Keratocan-IRES2-nls-Cre knock-in mouseのバイオイメージング (2015)
  • Author(s): 林康人
  • Organizer: 第119回日本眼科学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2015-04-16 – 2015-04-19
• [Presentation] Keratocan-IRES2-nls-Cre knock-in mouseのバイオイメージング (2015)
  • Author(s): 林康人
  • Organizer: 第119回 日本眼科学会総会
  • Place of Presentation: 札幌市教育文化会館 (北海道、札幌市）
  • Year and Date: 2015-04-16