Bone Marrow Mesenchymal Cell Transplantation for thin Corneal Stroma
Project/Area Number |
26462690
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Ophthalmology
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Research Institution | Ehime University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
林 康人 愛媛大学, 医学部附属病院, 講師 (70314953)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | ケラトサイト / バイオイメージング / 骨髄線維芽細胞 / 再生医療 / 細胞増殖 / Fucci / Creレポーターマウス / 骨髄細胞 |
Outline of Final Research Achievements |
Bone Marrow Mesenchymal Cell Transplantation for thin Corneal Stroma ROSA26 (CAG-loxP-NeoR-loxP- mVenus -hGem (1/110) IRES2 mCherry -hCdt1 (30/120) ) knock-in mouse lines were newly developed by gene targeting technique using C57BL/6-derived ES cells for tissue specific cell cycle bio-imaging. These mice were mated with Keratocan-IRES2-nls-Cre to get bone marrow mesenchymal cell culture (BMC) . The BMCs were injected into artificial thin cornea stroma. The transplanted BMC proliferation were observed a confocal microscope or a multi-photon microscope in-vivo or ex-vivo. Corneal stromal opacities were appeared two weeks after BMC injection. Over-proliferation of BMCs increase the thickness of corneal stroma, but ruin its clarity.
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Report
(4 results)
Research Products
(3 results)